Calculate your dilution strategyto make a 625 ng/mLHRP solution from a 12.5 μg/mL stock given the totalvolume of enzyme youwillneed for your condition this week. Note if you are testing pH, you only need to calculate one enzyme dilution because all other pHs will use the same strategy.
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Calculate your dilution strategyto make a 625 ng/mLHRP solution from a 12.5 μg/mL stock given the totalvolume of enzyme youwillneed for your condition this week. Note if you are testing pH, you only need to calculate one enzyme dilution because all other pHs will use the same strategy.
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- An enzyme catalysed reaction has a Km of 8 mM and a Vmax of 13 nM.s-1. Use the Michaelis-Menten equation to calculate the reaction velocity when the substrate concentration is 18 mM.An enzyme is present at 100 nM (nanomolar) and has a Vmax value of 25 uM/s (micromolar/second). The Km for the substrate is 5.2 uM. What is the kcat value? Report your answer to three significant figures in units of 1/s."Enzyme immobilization confers greater stability to the enzyme"-Do you agree or disagree with the statement? With appropriate example justify your opinion. Why the material used to immobilize the enzyme must be inert?
- How many moles of active sites are there in 1 mg of enzyme? Assume that each subunit has one active site.True or False Immobilization improves the stability of the enzyme. EnaLne, has a half-life of 10 days in free solution, but under identical conditions of temperature, pH, and medium composition, the measured half-life of a packed column is 30 days. The enzyme is immobilized in a porous sphere 5 mm in diameter.In a biochemical reaction that involves the substrate urea and the liver enzyme urease, decreasing the pH by one unit from its optimum value will _______ the rate of enzyme activity.
- NOTE: the enzyme-inhibitor complex requires 450 joule/mol to dissociateJOULE/MOLEnzyme specificity (list the different types of enzyme specificities)As an intermediate step in making the enzyme solution, you need to prepare Buffer X using the stock solutions of each chemical. Describe how you would make 100 mL of buffer X that has the following concentrations and pH: 50mM NaCl, 20mM Tris, 5mM MgCl2, pH 7.5 You do this starting with stock solutions of 1M NaCl, 1M Tris, 1M MgCl2, and water and a pH meter.
- Why can’t an enzyme have a kcat/KM value greater than 109 M−1 ∙ s−1?For an enzyme kinetics experiment, a student prepared a reaction mixture by mixing 450 microliters of 0.75mM PNPP with 4.25ml of 0.2M Tris-HCl buffer. When he is ready to measure the absorbance, he added 0.3ml of Alkaline Phosphatase to the mixture and mixed thoroughly. What is the substrate concentration at the beginning of the reaction in mM ?Describe how you would make the following solution containing two enzymes. 1 mL total volume in Buffer X with 10nM of enzyme A and 50nM of enzyme B You are given a solution of enzyme A at 1 mg/mL and a solution of enzyme B at 1 mg/mL. The molecular weight of enzyme A is 75,000 g/mol and the molecular weight of enzyme B is 130,000 g/mol.