For the following amino acid and dipeptide: Phe and His-Thr a. Draw the protonic equilibria b. Predict the predominant ionized forms at pH 7 c. Calculate the IpH
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For the following amino acid and dipeptide:
Phe and His-Thr
a. Draw the protonic equilibria
b. Predict the predominant ionized forms at pH 7
c. Calculate the IpH
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- Based on the given figure,a. where is the region or point where AA is predominantly present as a (-2) charged speciesb. where is the effective buffering range for the amino acid on the acidic regionIn the table below, name each functional group in anserine that is ionizable and record an estimated pKaIn case of the same amino acid as in the picture, in which ionization state would this amino acid be at (i) a pH that will correspond with the pI-value and (ii) a pH below the pI-value?
- The side chain of acetyl-L-lysine-OMe has a pKa=10.5 and its acid-dissociation reaction is shown below. see attachment for Question D.List all the amino acids you would expect to bind to a cation-exchange column at pH 2.For the amino acid tryptophan: Draw its complete protonic equilibria. Indicate the net charge of each form and encircle the zwitterionic form. Provide labels (A, B, C, etc.) for each form. b. Calculate the IpH of the amino acid. c. At what pH range(s) it can act as a buffer? d. What will be the predominant form(s) of the amino acid at pH (i) 1.5; (ii) 3.0; (iii) 4.5; (iv) 6.0; (v) 7.5 and (vi) 9.0?
- Given a tripeptide Cys-His-Lys, Cys: Pk1 = 1.5; Pk2 = 10.8; PkR = 8.5 His: Pk1 = 1.6; Pk2 = 9.0; PkR = 7.0 Lys: Pk1 = 2.2; Pk2 = 8.5; PkR = 9.8 a.draw the protonic equilibria for the tripeptide. b.what is the IpH? c.What is the dominant structure at pH 3.0? d.What is the first buffering region of the tripeptide?Using the Nernst equation, calculate the equilibrium potential for Ca2 and for C1 from the following sets of data: a. Given [ Ca2+ ]0=1mM,[ Ca2+ ]i=100nM, find Eca2+ b. Given [ Cl- ]0=110mM,[ Cl- ]i=100mM, find Ecle) What would be the predominant ionization states of this amino acid at physiological pH (7.4) and at this pH, what would the ratio of these two states be (show the calculation)?
- A 0.0284 M aqueous solution of lactic acid, HC3H5O3, a substance that accumulates in the blood and muscles during physical activity, is found to be 6.7% ionized. Determine the value of Ka for lactic acid.If an octapeptide IDEWLVAS is digested with chymotrypsin enzyme, which column would you use? Chymotrypsin cleaves on the carboxy side of bulky and aromatic amino acids. A. Sephadex column B. None of the above C. Anion Exchange column D. Cation Exchange column Please explain why.Sketch the following two tripeptide chains: Asp-Gly-Ser and Ile-Asn-Met at physiological pH . Please provide only typed answer solution no handwritten solution needed allowed