Cancer cells were characterized for their high transcriptional and translational levels. Direct modification for the gene of interests during transcription could be complicated and troublesome. Moreover, simply knocked down a certain gene could stimulate other compensating pathways. Fortunately, there are many controls for gene transcription. Modifying on these controls mechanisms could be easier and more effective compared to changing the gene of interests directly. If you are working in a biotech company, please suggest three possible sites/targets to modify in order to decrease a certain gene transcriptional level in cancer cell.

Cancer cells were characterized for their high transcriptional and translational levels. Direct modification for the gene of interests during transcription could be complicated and troublesome. Moreover, simply knocked down a certain gene could stimulate other compensating pathways. Fortunately, there are many controls for gene transcription. Modifying on these controls mechanisms could be easier and more effective compared to changing the gene of interests directly. If you are working in a biotech company, please suggest three possible sites/targets to modify in order to decrease a certain gene transcriptional level in cancer cell.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter9: Gene Expression And Gene Regulation

Section: Chapter Questions

Problem 10QP: The pre-mRNA transcript and protein made by several mutant genes were examined. The results are...

See similar textbooks

Similar questions

A strain of bacteria possesses a temperature-sensitive mutation in the gene that encodes the rho subunit. At high temperatures, rho is not functional. When these bacteria are raised at elevated temperatures, which of the following effects would you expect to see? Explain your reasoning for accepting or rejecting each of these five options. a. Transcription does not take place. b. All RNA molecules are shorter than normal. c. All RNA molecules are longer than normal. d. Some RNA molecules are longer than normal. e. RNA is copied from both DNA strands.
Transcriptional repressor proteins (e.g., lac repressor), antisense RNA, and feedback inhibition are three different mechanisms that turn off the expression of genes and gene products. Which of these three mechanisms will be most effective in each of the following situations? A. Shutting down the synthesis of a polypeptide B. Shutting down the synthesis of mRNA C. Shutting off the function of a protein For your answers to parts A–C that list more than one mechanism, which mechanism will be the fastest or the most efficient?
Regarding transcriptional promoter sites, which of the following statements are true? Select one or more than one: a)They are located in the gene (DNA) whose information will be transcribed b)They are found at the 3 'end of the gene that will be transcribed c)Some of them are called 'TATA box' d)They are found in the DNA, 'upstream' of the gene to be transcribed. e)They are proteins of the cytoplasm
For each statement about gene expression mechanisms, choose the correct end to the sentence. For each gene, the template strand for transcription is determined by…. The direction of translation is determined by…… The tissue-specificity of protein production is determined by…. choices: a. location of the start codon b. location of the promoter c. direction of polymerization by RNA polymerase d. none of these e. direction of movement of ribosomes f. overall orientation of the chromosome
Which of the following is least related to the other items? Group of answer choices inducer repressor operator enhancers 2. A scientist observing a cell during gene expression would be able to easily distinguish it as a prokaryotic cell by which of the following observations? Group of answer choices as soon as the DNA introns are removed from the template after the 5' caps are converted to mRNA after a transcription initiation complex has been formed during transcription once the pre-mRNA has been converted to mRNA
Numerous diseases are treated by injection of polypeptide growth factors, such as human growth factor. A project team in a pharmaceutical company wishes to produce high levels of a growth factor for therapeutic use by overexpressing and purifying the protein from E coli. The team takes the cDNA of the human gene and makes the mutations below before transferring the gene to E coli for high level protein expression. As seen in the gel below, the mutations dramatically enhanced the levels of protein produced (growth factor indicated by arrows). Explain why the team altered the human cDNA of the growth factor as shown below for expression in E coli (limit 3-4 sentences).
You are studying a new gene that is expressed within the adipose tissue of humans during the development of diabetes. Specifically, you want to learn why gene expression levels are very high in those people who develop diabetes. What DNA sequence would you concentrate on? Group of answer choices A specific response elements B the TATA box C enhancer sequences D promoter proximal elements E metal response elements
Enhancers are sequences that affect the initiation of the transcription of genes that are hundreds or thousands of nucleotides away. Transcriptional activator proteins that bind to enhancers usually interact directly with transcription factors at promoters by causing the intervening DNA to loop out. An enhancer of bacteriophage T4 does not function by looping of the DNA (D. R. Herendeen et al. 1992. Science 256:1298–1303). Propose some mechanisms other than DNA looping by which this enhancer might affect transcription at a gene thousands of nucleotides away
You are a genetic engineer that is working on transcriptional regulation in living organisms. In addition to your day job, you are a video game connoisseur and are designing a cell phone game where participants design the features of an organism that is the best at up-regulating transcription of a gene, WINNING. Participants are able to pick from a variety of organisms, cis regions, trans factors and a variety of enzymes. You want to set the high score on the leader board so you play the game and design the following features into your organism. - You need to describe ALL the features you will be including into your organism as well as what organism you picked for your entry. You need to include all the components of transcription, key features of transcriptional regulation and other features that will increase transcription to the highest level. You need to explain why you included every feature and how each will contribute to increased gene expression. Be creative and error on the…
Regulation of transcription is mediated by proteins that first must bind to specific sequences/elements that are present in DNA. Fill in the table below based on your knowledge of the different sequences and proteins involved in the regulation of eukaryotic gene expression. Regulatory sequence Category of Protein that Binds Effect on Transcription of Associated Gene Generic or Gene-Specific Regulation? Core Promoter Element Activator Silencer
Give one example of a prokaryotic system of gene expression that is more complicated (duringtranscription at the promoter) than its corresponding eukaryotic system of gene expression.A. prokaryotes require general transcription factors to initiate transcriptionB. prokaryotes typically have activator and repressor DNA sequences thousands of nucleotides awayC. prokaryotes have three classes of RNA polymerasesD. prokaryotes always have their DNA tightly packed into higher-level structures than nucleosomesE. prokaryotes usually have polycistronic mRNA
A bacterial species has a hypothetical sigma promoter that has the following sequence: TTGGCA - 18 bases - TATAAT What change in the level of transcription would there be if the sequence was mutated to: TTCGCA -18 bases -TATAAT 1.The mutation would move the promoter away from consensus and reduce the level of transcription 2.No change the consensus TATAAT sequence in the same. 3.The mutation would bind the promoter to the consensus and produce normal levels of transcription 4.The mutation would inhibit the promoter thereby inhibiting transcription