S-CCT CCT C The protein assay kit that we will use to quantitate horseradish peroxidase requires absorbance measurements at 562 nm. What type of radiation is this? 6.
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- 42. What is the distinction between plectonemic and solenoidal supercoiling? Which of the two is more stable in the absence of protein? Which form of supercoiling is found within cells and why?How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein purification techniques?what are the advantages and disadvantages of using recombinant protein and affinity chromatography for protein purification compared to gel filtration (size exclusion chromatography) and DEAE-sepharose chromatography (ion-exchange chromatography)?
- Why were radioactive sulfur and phosphorous used to label bacteriophage in Hershey and Chase’s experiments?Using the SDS-PAGE and the calibration curve given, calculate the size of the lysozyme and ovalbumin bands from this lysozyme purification. Lysozyme is the bottom band in the samples, ovalbumin is the most intense band in the first three samples, and the first and last lanes are molecular mass standards.With an A260 value of 1.12 and a DNA concentration of 56.0 ng/mL, what is the pathlength of the optical system used within the Nanodrop?
- During incubation, prepare dilutions of the standard antiserum which constitutes the standard curve for the assay (concentration in ng/mL). Add 500 µL of PBS-milk to the supplied microtube containing 500 µL of standard antiserum to obtain Standard 1 [500 ng/mL]. Identify seven microtubes for standards 2 to 7 and place 500 µL of PBS-milk in each. Calculate how much of antiserum is used in each standard ?Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays, what can be done if a concentrated sample is out of the detection range of chosen dye-based assay?You have an extract of BL21(DE3) cells containing a soluble, 75 kDa recombinant protein that has a histidine tail at its C-terminus. This extract is still in raw form and you need to purify this protein without losing its solubility. Check the option below that represents the best purification methodology in the order of execution. * a) (1) isoelectric precipitation; (2) microfiltration; (3) affinity chromatography; (4) anion exchange chromatography b) (1) centrifugation; (2) microfiltration; (3) isoelectric precipitation; (4) affinity chromatography c) (1) centrifugation; (2) microfiltration; (3) affinity chromatography; (4) ultrafiltration d) (1) microfiltration; (2) jumping out; (3) centrifugation; (4) affinity chromatography
- Quinolone antibiotics treat bacterial infections by blocking the activity of topoisomerase. Why does this treatment work? Explain what occurs at the molecular level.35. In five Hfr strains, each of which was used to build a time-of-entry map, the genes entered the recipient cells as follows: Strain 1: N O S S N A Strain 2: A P T H A N Strain 3: P T H A N O Strain 4: A N O S S N Strain 5: N A P T H A Build the map from this data and enter your result.What volume of 4X loading buffer must be added to 21 micro L of DNA in a technique for DNA sample preparation for agarose gel electrophoresis to generate a 1X buffer solution?