Change the procedure to past tense and predict the result

Biology: The Dynamic Science (MindTap Course List)
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Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
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Chapter37: Plant Signals And Responses To The Environment
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Change the procedure to past tense and predict the result 

1. Root initiation
Soak 30 seeds of common garden bean, Phaseolus vulgaris, for about 1 hour in tap water in a beaker.
Plant the seeds well apart and about 0.5 cm deep in a small, paper-lined flat of sand. Water the sand
thoroughly; then germinate in the dark at 25°C for 5 days, until the hypocotyls begin to show. Trans-
fer the flat to the green-house, and grow for an additional 7 days, until the plants have formed a pair
of simple leaves. Completely cover the outside of plastic vials (2-inch in diameter) aluminum foil. Tie
a tag round. each jar neck with string. Fill each jar with 100 ml of one of the following solutions, and
label:
1.
Distilled water.
2. 0.1 mg indoleacetic acid per liter.
1.0 mg indoleacetie acid per liter.
3.
With a sharp razor blade, excise each bean plant at the level of the earth, remove both cotyledons,
cut the hypocotyl 5 am long, as measured from the point of cotyledon attachment to the out base.
Immediately put the hypocotyl into the solution, with the pair of leaves projecting above the lid and
the cotyledonary stumps below the lid. Repeat the procedure, 1 plant at a time, working rapidly to
avoid drying of the plants, until 5 plants have been placed in each vial. Select plants as similar as
possible. Place the vial in a row on the shelf above your laboratory bench.
One week later, make the following measurements on each hypocotyl:
1. Number of rows of lateral roots.
2. Number of lateral root primordia (shorter than 1 mm) in each row.
3. Length of lateral roots in mm.
Determine the averages for each treatment, and summarize the results in the table on your report
sheet.
Transcribed Image Text:1. Root initiation Soak 30 seeds of common garden bean, Phaseolus vulgaris, for about 1 hour in tap water in a beaker. Plant the seeds well apart and about 0.5 cm deep in a small, paper-lined flat of sand. Water the sand thoroughly; then germinate in the dark at 25°C for 5 days, until the hypocotyls begin to show. Trans- fer the flat to the green-house, and grow for an additional 7 days, until the plants have formed a pair of simple leaves. Completely cover the outside of plastic vials (2-inch in diameter) aluminum foil. Tie a tag round. each jar neck with string. Fill each jar with 100 ml of one of the following solutions, and label: 1. Distilled water. 2. 0.1 mg indoleacetic acid per liter. 1.0 mg indoleacetie acid per liter. 3. With a sharp razor blade, excise each bean plant at the level of the earth, remove both cotyledons, cut the hypocotyl 5 am long, as measured from the point of cotyledon attachment to the out base. Immediately put the hypocotyl into the solution, with the pair of leaves projecting above the lid and the cotyledonary stumps below the lid. Repeat the procedure, 1 plant at a time, working rapidly to avoid drying of the plants, until 5 plants have been placed in each vial. Select plants as similar as possible. Place the vial in a row on the shelf above your laboratory bench. One week later, make the following measurements on each hypocotyl: 1. Number of rows of lateral roots. 2. Number of lateral root primordia (shorter than 1 mm) in each row. 3. Length of lateral roots in mm. Determine the averages for each treatment, and summarize the results in the table on your report sheet.
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