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Answers with full explanations
The diagram below represents a lab setup. The artificial cell (dialysis tube) contains a
starch solution and the beaker contains a solution of starch indicator and water. The setup is
lefe undisturbed for twenty minutes.
Stárch indicator and water
-Dialysis tube
Starch solution
Identify one molecule, present in this setup, that will be able to pass through the dialysis tubing. [1]
Describe one observation that could be made that would confirm that the molecule
question 77 had passed through the membrane. [1]
you
identified in
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- A. a pre-weighted dialysis bag which contained a solution of 10% glucose was placed in a beaker which contained 20% glucose. After one hour, the bag was weighted again. Calculate the % mass change of the dialysis bag from the following information: Mass of bag before the experiment: 15.3g Mass of bag after the experiment: 12.7% B. was the beaker solution hypertonic, hypotonic, or isotonic?There are 3 dialysis tubing each containing 8ml of 20% sucrose solution.#1 tubing was immersed in distilled water.#2 tubing wad immersed in 20% sucrose solution while the third tubing was immersed in 40% sucrose solution.what will happen to each dialysis tubing and why it occura. What is the function of the SDS in the lysis buffer? the NaOH? b. What does the potassium acetate do in the procedure and why?
- Refer to the chromatograph (Figure 1) below and answer the questions that follow: What is the volumetric flow rate of the column? What type of compound is likely to be found in peak B? Explain. What possible buffer changes could the researcher have applied at the 30ml mark? Explain. How would one avoid cross-contamination when using this type of purificationExplain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #’s from the incubated plates. ……A serial dilution yielded TNTC in the 1:1 plate and the 1:10 plate, 50 cfu’s in the 1:100 plate and 3 cfu’s in the 1:1000 plate . What is the concentration (CFU)/ml of the raw sample? Is water acceptable for potable or Recreational use? Explain. When do you use filtration rather than serial dilutionIf there are 103 cells per mL at the middle of log phase, andthe generation time of the cells is 30 minutes, how many cellswill there be 2 hours later?a) 2 x 103b) 4 x 103c) 8 x 103d) 1.6 x 104e) 1 x 107
- The practice question i need help with explains that we need to Obtain a 5 cm piece of dialysis tubing. Clamp one end of tubing. Fill the tube with sugar/starch solution, clamp the other end. This is your “cell”.Weigh cell and record weight. Place your cell in beaker of 100% water. Wait 1 hour. After, Re weigh your cell and record weight. Test water in beaker for the presence of sugar and for presence of starch using the standards procedure from Lab 2. Initial weight ________________g Weight after 1 hour __________________g Was your cell in a hypotonic, hypertonic, or isotonic solution? How do you know? Did sugar diffuse out of the cell? Did starch diffuse out of the cell? What is the relationship between size and diffusion across a semipermeable membrane?46 50 52 60 41 46 55 Find the ff. WITH SOLUTION PLEASE MeanMedianModeSDVarianceCVQuartile 2Decile 7Percentile 25Percentile 30In biological systems, buffers are important because they facilitate changes in pH to enhance biological activities of proteins and nucleic acids. True False
- Hemodialysis is a process by which a machine is used to filter urea and other waste products from an individual’s blood when the kidneys fail. The concentration of urea in the blood is often modeled as exponential decay by the following function: −Kt c(t)=c0e V where K is the mass transfer coefficient (in mL/min), c(t) is the urea concentration in the blood at time t (in mg/mL) and V is the blood volume., and c0 is the initial concentration at time t = 0. Answer the following questions:(a) How long should a patient be put on dialysis to reduce the blood urea concentration from an initial value of 1.65 mg/mL to 0.60 mg/mL, given that K = 340 mL/min and V = 32, 941 mL? b) Derive a general formula for the dialysis time T , in terms of the initial urea concentration c0 and he target urea concentration c (T ) (c) The quantity Kt, is sometimes used as a measure of dialysis treatment adequacy. What are its V units and explain what does this quantity represent?With the principles of GFC, which of the following statements is incorrect?a. For small molecules, their elution volume, Ve > VOb. For a very large molecule, its elution volume, Ve = Voc. Blue dextran is used to determine the void volume because it is too large and cannot get into the beads and therefore excluded from the gel.d. Low molecular weight molecules are freely accessible to the gel particles, and elute at a volume equal to the internal pore volume.In an SDS-PAGE experiment, protein X has an Rf = 0.75, while protein Y has an Rf = 0.30. Which of the following statements are FALSE during the electrophoretic run?I. X has a greater charge-to-mass ratio than Y, since X has a smaller MW.II. X has a lower MW, and thus experiences less friction with the gel matrix.III. X has more hydrophobic groups, thus is in a more linearized state.IV. X is globular, while protein Y is rod-like, thus X has higher mobility.V. X is affected by less magnitude of electric field because of smaller…In this experiment, you are to prepare 250mL of Cell Lysis Buffer using the reagents given in Table. Fill out the table. Show your computations at the bottom of the table.