CH=N+=N- C=0 CH2 CH, H-C-NH3* COO
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
6-Diazo-5-oxonorleucine (DON) irreversibly inhibits glutamine-dependent amidotransferases.
(a) Which intermediate or intermediates in purine
would you expect to accumulate in DON-treated cells?
(b) Which participants in pyrimidine synthesis would you expect to
accumulate?
(c) Speculate on the mechanism by which DON inhibits these enzymes.
Introduction:
DON is a water-soluble glutamine ally that was separated initially from Streptomyces in an example of Peruvian soil. It is a non-standard amino acid.
Explanation:
a.
The intermediates in purine nucleotide biosynthesis that accumulated in DON-treated cells are:
- Xanthosine monophosphate (XMP)
- Phosphoribosyl pyrophosphate (PRPP)
- Formyl glycinamide ribonucleotide
b.
Uridine triphosphate (UTP) is the participant in pyrimidine synthesis. These are pyrimidine ribonucleotides with a triphosphate bunch group to the ribose part.
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