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Serial dilution is recommended prior to pour and spread plate. Why is this so?
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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?Describe the type of media you choose in the laboratory, use and prepare them ,, I want to describe how to prepare the media ,, smear from the cheek
- What is one advantage of a pour plate over a streak plate?What is the difference between the pour plate method and the spread plate method? Why might you choose one over the other? What kind of plate must this be and why do you say that?What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
- What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateDuring the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture is discarded in the disinfectant solution. Why?Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%
- Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise when the slide is heated in a flame?To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. How did you calculate it?. Single line text.
