Test Description Terminal Terminal Medium, Incubation Incubation Results temperature time (°C) electron enzyme reagent ассеptor Oxidase test Tryptic soy agar (TSA), OxiDrop 1 24 – 48 hours Catalase Oxygen test N/A Nitrate reductase 3. 37 N/A test
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- Activity 13 Urine Culture Inoculating Urine with a calibrated loop The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures. PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…Please explain and cite references if possible. Thank you! 1. Explain why stool specimen must not be frozen nor placed in incubators.Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.
- QUALITATIVE TESTS: Biuret Test Ninnhydrin TestXanthoproteic TestMillon's TestHopkin's-Cole TestSakaguchi TestNitroprusside TestFohl's TestTest for AmidePauly's Testorder: ticargram 750 mg IM every 6 hours direction for reconstitution: add 2 mL of sterile water for injection; each 2.6 mL of solution will contain 1g of ticarillin how many millimeters will be administered for each doseCause and Effect Analysis describe the effect on the test procedure and explain briefly The zones of adjacent antibiotic disks overlap. Antibiotic disk cartridge was left open for more than 7 days. Antibiotic disk was placed closely at the edge of the plate.
- Order: cefoxitin sodium (Mefoxin) 200 mg IM Q4HSupply: 1g powderDiluent: 2 mL sterile waterConcentration 400 mg/mLRound to the nearest tenth Order: 1000 mL D5NS IV to run at 150 mL/hrCalculate the drops per minute (gtts/min) for each of the different tubing.a) Macrodrop (10 gtt/mL) b) Microdrip (60 gtt/mL)Paraphrase the text below: Series of test tubes were filled with the desired volume of the BSA (0.1, 0.2, 0.3… 1 ml) .PBS was added to this to make the volume of 1 mL. 5 mL of copper reagent was added to all the tubes. After proper mixing, all the tubes were incubated at room temperature for 15 minutes. 1 mL Folin reagent was added to each and mixed properly with the help of vortex mixer and incubated for 20 minutes. The intensity of the colour was then determined spectrophotometrically at 680 nm. The graph was than plotted between optical density and the amount of BSA. Proteins estimation was done for the detection for the amount of proteins present in the sample solutions by the Lowry methodDILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?