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Complete the following problems. Restriction enzymes (REs), which cut D NA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, PMBBS, through restriction digests. You subjected the PMBBS plasmid to complete digestion by different combinations of three REs (EcoRI, BamHI, and Xhol), and analyzed the results on an agarose gel, shown below. Using the data you can glean from this gel, answer the questions that follow. EcoRI BamHI Xhol DNA size ladder 3000 bp 2500 bp 2000 bp 1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp "The same total amount of DNA was loaded in each lane. vC 100bp Plus DNA ladder from Vivantis Technologies 1. What is the total size of the PMBBS plasmid in bp? Answer: bp 2. How many cut sites on the PMBBS plasmid does each RE have? EcoRI: BamHI: Xhol: 3. If the PMBBS plasmid were digested with all three REs together, how many fragments would be observed, and what would be the sizes (in bp) of these fragments? Number of fragments: Sizes:

Complete the following problems. Restriction enzymes (REs), which cut D NA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, PMBBS, through restriction digests. You subjected the PMBBS plasmid to complete digestion by different combinations of three REs (EcoRI, BamHI, and Xhol), and analyzed the results on an agarose gel, shown below. Using the data you can glean from this gel, answer the questions that follow. EcoRI BamHI Xhol DNA size ladder 3000 bp 2500 bp 2000 bp 1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp "The same total amount of DNA was loaded in each lane. vC 100bp Plus DNA ladder from Vivantis Technologies 1. What is the total size of the PMBBS plasmid in bp? Answer: bp 2. How many cut sites on the PMBBS plasmid does each RE have? EcoRI: BamHI: Xhol: 3. If the PMBBS plasmid were digested with all three REs together, how many fragments would be observed, and what would be the sizes (in bp) of these fragments? Number of fragments: Sizes:

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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For this question please can I have sketch of a map of the pMBBS plasmid showing

the relative location of RE sites

size in base pairs of segments of DNA between sites 

il MTN 5:41 AM O 28% mplete the following problems. striction enzymes (REs), which cut D NA at specific sequences, are classic tools in logy. Because of their specificity in cutting DNA, REs can be used to "map" DNA s analyzing the fragments generated upon restriction digest, as in the example shown Your task is to study the circular plasmid, pMBBS, through restriction digests. You PMBBS plasmid to complete digestion by different combinations of three REs (EcoR d XhoI), and analyzed the results on an agarose gel, shown below. Using the data you m this gel, answer the questions that follow. EcoRI BamHI Xhol DNA ladder 3000 bp 2500 bp 2000 bp 1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp *The same total amount of DNA was loaded in each lane. "vC 100bp Plus DNA from Vivantis Techn What is the total size of the pMBBS plasmid in bp? swer bp How many cut sites on the pMBBS plasmid does each RE have? DRI: BamHI: XhoI: If the pMBBS plasmid were digested with all three REs together, how many fragmer observed, and what would be the sizes (in bp) of these fragments? mber of fragments: zes: bartleby.com +||
Transcribed Image Text:il MTN 5:41 AM O 28% mplete the following problems. striction enzymes (REs), which cut D NA at specific sequences, are classic tools in logy. Because of their specificity in cutting DNA, REs can be used to "map" DNA s analyzing the fragments generated upon restriction digest, as in the example shown Your task is to study the circular plasmid, pMBBS, through restriction digests. You PMBBS plasmid to complete digestion by different combinations of three REs (EcoR d XhoI), and analyzed the results on an agarose gel, shown below. Using the data you m this gel, answer the questions that follow. EcoRI BamHI Xhol DNA ladder 3000 bp 2500 bp 2000 bp 1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp *The same total amount of DNA was loaded in each lane. "vC 100bp Plus DNA from Vivantis Techn What is the total size of the pMBBS plasmid in bp? swer bp How many cut sites on the pMBBS plasmid does each RE have? DRI: BamHI: XhoI: If the pMBBS plasmid were digested with all three REs together, how many fragmer observed, and what would be the sizes (in bp) of these fragments? mber of fragments: zes: bartleby.com +||
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