Briefly describe the function of each of the following components of the DNA extraction protocol. Please consult the practical manual. (Students taking the online alternative, derive these steps from the practical manual and BI101JC_Biochemistry Practical DNA PROTEIN, or from the Connect Virtual Lab on DNA Extraction). i. Heat ii. Protease Solution (protein-digesting enzymes in meat tenderizer) iii. Detergent (emulsifier) iv. Salt v. Ethanol

Q: DNA extraction, PCR, gel electrophoresis, and DNA sequencing of PCR products. Give a description of…

A: DNA extraction is the procedure of isolating the Deoxyribonucleic acid from biological samples. PCR…

Q: The red line indicates where a radioactively labeled probe hybridizes. Three samples of the genomic…

A: P                      B                         PB…

Q: 1000bg 800b 500bd 300bd 100bg Identify the sizes of the DNA bands in the agarose gel above using the…

A: Agarose gel electrophoresis is a separation technique mostly used for DNA molecules. Where a sample…

Q: 1d) Whether done manually or automated, DNA sequencing gels are always made of polyacrylamide rather…

A: Deoxyribonucleic acid (DNA) stores the cell’s genetic information and is present in the nucleus of…

Q: Tm value, the GC-content of the selected DNA sequence and the absolute nucleotide composition of the…

A: DNA stands for deoxyribonucleic acid and it consists of 4 types of nitrogen bases, adenine, guanine,…

Q: Figure 9-22 shows the first steps in the process of making a DNA microarray, or DNA chip, using…

A: A DNA microarray is also known as the DNA chip, is the high throughput method that is used to trace…

Q: Determine the effect (increase, decrease, no effect or cannot be determined) of the stated condition…

A: DNA is Deoxy ribonucleic acid’s and DNA have sugar phosphate as backbone and this sugar phosphate…

Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…

A: DNA (Deoxyribonucleic acid) and RNA are two examples of nucleic acids (Ribonucleic acid).…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: The central dogma is DNA --> mRNA --> Protein. Hence, any mutations in the DNA lead to changes…

Q: consider the DNA segment with a sequence: 3'-TACGGTACGGGATTG-5'. if the given DNA sample was…

A: Ion Torrent sequencing is also known by the name of: Ion semiconductor sequencing pH-mediated…

Q: Which of the following gels is the preferred choice for separation of DNA fragments?  Agarose…

A: There are several techniques which are used in recombinant DNA technology. In a recombinant DNA…

Q: What are the roles of the following reagents in DNA extraction? a. Ethanol b. NaCl c. SDS d. TE…

A: A) The initial role of the ethanol and monovalent cations is to remove the solvation shell…

Q: A DNAR document is legally binding. True or False?

A:

Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…

A: The corner stone of genetic engineering or recombinant DNA technology is a special class of enzymes…

Q: Specify the method for Purification of DNA fragment after digestion.

A: Dna is the polymer formed by monomer units called nucleotides . The dna molecule when degraded…

Q: You have a very concentrated sample of DNA which you need to dilute for sequencing 1:6000 with a…

A: Dilution is defined as the ratio of volume from stock to the final volume.  Dilution= Stock volume /…

Q: Select all the following statements that are correct regarding agarose gel electrophoresis and…

A: Agarose gel electrophoresis is a process used for separation of DNA fragments. Steps of agarose gel…

Q: In the Sanger (dideoxy) method for DNA sequencing, a small amount of a dideoxynucleoside…

A: Sanger sequencing, also termed chain -terminator sequencing process is based on the ability of the…

Q: A DNA solution of unknown concentration was subjected to quantitative analysis. For better accuracy,…

A: The DNA concentration of a solution can be calculated by taking absorbance at 260nm as DNA absorbs…

Q: What is the purpose of adding the dishwashing liquid to the DNA extraction?

A: DNA extraction is a method of separating DNA from cell membranes, proteins, and other cellular…

Q: One micromole of 48-nucleotide(nt) DNA was synthesized via solid phase synthesis. This sample…

A: DNA or deoxyribonucleic acid is a polynucleotide chain made of monomeric units of nucleic acids.…

Q: Complete the following problems. Restriction enzymes (REs), which cut D NA at specific sequences,…

A: Answer ::   Introduction A plasmid is a genetic structure in a cell that can replicate independently…

Q: Describe two methods in performing a DNA analysis, its possibleapplications, and how it can be so…

A: DNA (Deoxyribonucleic acid) analysis is a method of determining the characteristics of the…

Q: What are the DNA extraction methods? Explain each one.

A: Ans: The Deoxyribonucleic acid (DNA) needs to be extracted out of cell for further studies on which…

Q: Complete the following problems. Restriction enzymes (REs), which cut D NA at specific sequences,…

A: Introduction A plasmid is a genetic structure in a cell that can replicate independently of its…

Q: N

A: Recombinant DNA , molecules of DNA from two different species that are inserted into a host organism…

Q: To ensure a conviction, DNA crime labs use CODIS standards. Instead of 1 tandem repeat, how many…

A: Forensic science is concerned with the collecting and examination of physical evidence as well as…

Q: What are some difficulties with low copy number DNA analysis? What kinds of checks and balances can…

A: INTRODUCTION DNA analysis DNA analysis is a method of analyzing the characteristics of DNA. It is…

Q: Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencing,…

A: Although the techniques used are same, there is difference in the chemicals used by ThermoFischer.…

Q: ! (please help ASAP)

A: DNA is also known as deoxyribonucleic acid. DNA is composed of four types of nucleotides attached…

Q: Why evenly spaced sequence in chromatogram is an indication of a good DNA chromatogram?

A: The characteristics of an evenly spaced sequence are:-  Evenly-spaced peak, each being…

Q: Consider the DNA segment with a sequence: 3'-TACGGTACGGGATTG-5'. If the given DNA sample was…

A: Pyrosequencing is a form of sequencing that works by the principle of DNA replication. The template…

Q: A batch of this DNA is first fully digested by HpaI alone, then another batch is fully digested by…

A: The restriction enzymes clean the nucleotide fragment at specific sides called the restriction…

Q: From your knowledge about DNA microarray, answer the following: A- How DNA microarray is created?…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three subparts…

Q: A description of the principles of agarose gel electrophoresis of DNA.

A: INTRODUCTION:- Gel electrophoresis separates DNA on the basis of its charge and size in a solid…

Q: Discuss the use of gel electrophoresis for the separation of macromolecules (DNA, RNA and protein)…

A: In biotechnology the gel electrophoresis is routinely used technique that is used for the separation…

Q: proteins are denatured and eliminated during the DNA extraction process. how can we determine the…

A: Deoxyribonucleic acid (DNA) is the hereditary unit of life, which carries the genetic information in…

Q: Some recombinant DNA techniques depend on the specific hybridization (or annealing) between two…

A: The invention of recombinant DNA technology was carried out by Herbert Boyer & Stanley & N.…

Q: Consider the four extraction/purification methods .  a. Which extraction method would you use for a…

A: DNA isolated from various biological samples can be used for a vast array of downstream…

Q: Which of the following is the correct order in extracting the DNA?   I. Washing of DNA II. Tissue…

A: DNA act as genetic material in most of organism . It is two stranded , ladder like structure . DNA…

Q: In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is…

A: DNA isolation: The process of extraction of DNA may be broken down into five primary phases that…

Q: Much of the human genome consists of repetitious DNA. Describe the difference between microsatellite…

A: Some of the similarities between microsatellite and minisatellite are that both are type of tandem…

Q: Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the…

A: The correct option is b 5'CCATTGG3'. Sanger sequencing ( method of DNA sequencing and it involves…

Q: In order to determine the purity of a DNA sample. spectrophotometry can be carried out at _______ to…

A: DNA yield and purity is measured by absorbance. Absorbance is measured  by spectrophotometer.

Q: As a technique for detecting genetic variations, RFLP has substantial drawbacks. Name one such…

A: RFLP (Restriction Fragment Length Polymorphism) is a technique, which was invented by an English…