You need to amplify a gene from a source DNA for cloning in any cloning vector, which enzymes will be used to accomplish the task? Discuss role of each enzyme used for cloning a gene in step wise order.

Q: Remember that although there are many interesting ideas about genetic engineering of plants and…

A: Genetic engineering is a one of the modern technology to modify organisms, plants, crops & even…

Q: What can serve as footprints to identify genome sites that are or have been occupied by transposable…

A: A genome is a collection of an organism's genetic information.

Q: How would you use PCR-amplified oligonucleotide directed mutagenesis to create deletion of amino…

A: Subtilisin is a protease that is obtained from bacteria Bacillus subtilis. It belongs to the group…

Q: You are trying to clone a gene, You have successfully isolated it from the genomic DNA of an…

A: The term DNA cloning is associated with a process that plays an important role in making copies of a…

Q: The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy.…

A: Enzymes  Enzymes are the protein that use as catalysts in metabolic and catabolic reaction to fasten…

Q: If I clone a complete eukaryotic gene, including the eukaryotic promoter region, ligate it into a…

A: Transformation is the process in which a bacterium takes up DNA from the environment, that has been…

Q: Your cloning vector has restriction recognition sites for two restriction endonucleases, EcorI and…

A: Introduction Recombinant DNA technology enables us to construct DNA using the Recombination method.…

Q: To clone a bacterial lipase gene into the cloning vector pET28a, you know the full sequence of the…

A: For the ease of manipulation, vectors must be relatively small molecules. They must be capable of…

Q: What types of cells are used for cloning rDNA?

A: rDNA is called recombinant DNA which contains DNA from multiple sources. Recombinant DNA is it's the…

Q: For human genomic DNA what is the expected fragment size for high molecular weight DNA extracts?…

A: Gel electrophoresis is a technique used for the separation of proteins or nucleic acids based on…

Q: What is a cloning vector?   A. The DNA probe used to locate a particular gene in the genome.   B.…

A: Vector is an autonomously replicating genetic element used to carry a fragment of desired DNA into…

Q: Create a simple outline (yes, you can use bullets and outlines!) starting from plasmid DNA with your…

A: Bacterial transformation is a key step in molecular cloning, the goal of which is to produce…

Q: Give three important characteristics of cloning vectors.

A: Cloning is an important process in which bacterial cells containing recombinant DNA can be readily…

Q: You’re working in a research lab, and your current task is to clone the gene that codes for…

A: The cloning process is used to introduced a foreign DNA into the genome of a particular host…

Q: Explain how bacteria can be used to produce large amounts as a cheap source of protein. Your…

A: Transgenic microbes - If the characteristic of microbes ( Bacteria) is changed by incorporating the…

Q: You’re working in a research lab, and your current task is to clone the gene that codes for…

A: Recombinant DNA technology is one of most significant techniques of genetic engineering. It enables…

Q: See the restriction enzyme map below. The total DNA length is 1800 base pairs. If this DNA is cut…

A: An enzyme derived from bacteria that cuts DNA molecules at specified sequences is known as a…

Q: What are the essential elements of a plasmid cloning vector? Discuss the importance of each element…

A: Essential elements of plasmid cloning vector  Presence of Origin of replication for autonomous…

Q: robes for cloned genes use ____.   a. certain bacteria that glow when they take up the genes   b.…

A: Gene cloning: It is a procedure that makes researchers prepare several similar copies of gene-sized…

Q: The plasmid cloning vector pBR322 is cleaved with the restriction endonuclease PstI. An isolated DNA…

A: The following is the DNA information received by gel electrophoresis:   In lanes 1 and 2, each…

Q: Design the main steps of cloning experiment.

A: Microbial enzymes have been employed in a wide range of sectors, including chemical, agricultural,…

Q: There are two time gaps in the process of cloning.  What are they?  (ie. What do you have to wait…

A: Cloning is the process of the formation of identical copies of the genetic material of the organism.…

Q: What type of enzymes are used to “cut” desired DNA sequences for use in recombinant gene technology…

A: Note: As Per Guidelines, We Can Answer One Question At A Time. Ask Again To get rest answers.…

Q: Herbert Boyer and Stanley Cohen pioneered the technique of DNA cloning allowing genes to be…

A: Ans: Recombinant DNA: The DNA formed by cloning of foreign gene in to the vector plasmid is referred…

Q: Restriction enzymes and DNA ligase are key components when preparing cloning vectors. True or False.

A: Main components for preparation of cloning vector are :  Origin of replication Marker gene…

Q: Ehat  primer sets could be amplify the following DNA sequence?   AATACGTCGCATGGggatccttttttatgcatg

A: Amplification of DNA sequence is done by the polymerase chain reaction. With the help of DNA…

Q: The temperature at which the primers and target DNA hybridize may be changed to influence the…

A: Introduction Temperature changes have an impact on the response, which may be noticed at both high…

Q: During an Ames test, what does it mean when S. typhimurium is his+? A. The sample is completely…

A: Ames test is a biological assay used to evaluate the mutagenic potential of chemical compounds.

Q: In selecting target cells to receive a transferred gene in gene therapy, what factors do you think…

A: Gene therapy involves transferring of cloned genes into target cells by the process of recombinant…

Q: What is function of DNA ligase? Why bacteria serve as an excellent tool for recombinant DNA…

A: DNA replication is a process in which DNA makes copies of itself and this property of DNA is used in…

Q: For each of the following scenarios, indicate Yes (it is cloning) or NO (it is NOT cloning)…

A: Cloning means an organism is generated genetically identical from an existing organism through…

Q: The complete genetic information of a person is isolated from some of her cells, cut with…

A: Genetics is a part of science worried about the investigation of genes, genetic variety, and…

Q: A molecular geneticist hopes to find a gene in human liver cells that codes for an important…

A: Introduction :- A nucleic acid sequence is a series of bases denoted by a set of five letters that…

Q: Find a genetically modified organism of interest. What organism is the origin of the coding…

A: GMO stands for the genetically modified organism, a type of life form whose genome has been altered…

Q: Steps in PCR and Gene Cloning Propose two hypothetically modified or genetically engineered plants…

A: Science is humanity's greatest blessing. It has altered human existence; nothing finer could have…

Q: The exponential nature of PCR allows spectacular increases in the abundanceof a DNA sequence being…

A: Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in…

Q: Suppose that a human genomic library is prepared by exhaustive digestion of human DNA with the EcoRI…

A: No,  because most humans genes are much longer than 4kb.

Q: Following are four processes common to most cloning experiments:  a)    transforming bacteria b)…

A: A "clone" simply means that an object, cell, or whole organism has the same genetic makeup as the…

Q: Explain why a cloning vector usually includes a selectable marker.

A: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into…

Q: List and explain the steps of DNA cloning using pUC8.

A: Introduction Molecular cloning is a set of molecular biology experimental methods for assembling…

Q: CRISPR RNA (crRNA) in a bacterial cell: is a snippet of genetic material from a virus stored in a…

A: The CRISPR/Cas9 or clustered regularly interspaced short palindromic repeats and CRISPR-associated…

Q: a) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short…

A: Note: Solving answer only for part A as per the guidelines, repost remaining part, please. The…

Q: Describe the important features of cloning vectors. Explain the purpose of selectable markers in…

A: Cloning vector refers to small piece of deoxy ribonucleic acid (DNA), which are used to propogate…

Q: What does gene shuffling mean, how is it done? What is it used for? What advantages does it have in…

A: During the meiosis cell division at prophase I, a pairing of homologous chromosomes takes place at…

Q: transformation and CRISPR. In your own words, briefly describe two differences between these…

A:

Q: PCR errors during library amplification are one possible source of false positive results. If an…

A: PCR is a polymerase chain reaction. This is a biotechnology used to amplify the DNA copies.

Q: Restriction enzymes are used in laboratory manipulations to cut DNA; many of these enzymes can be…

A: Restriction enzyme - Restriction enzyme are also called as Restriction endonuclease. restriction…

Q: List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs…

A: Cloning vectors are small DNA pieces that are maintained stably in an organism. This vector can a…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.