Compound X is metabolised by two distinct primary metabolic pathways. In vitro metabolism studies have found that Pathway 1 has a KM of 5 µM and a Vmax of 50 pmol/min/mg protein, whereas Pathway 2 has a KM of 20 μM and a Vmax 5 pmol/min/mg protein. i) At a Compound X concentration of 0.1 µM, calculate the intrinsic clearance values for (a) Pathway 1, (b) Pathway 2, and (c) overall metabolism by these two pathways ii) At a Compound X concentration of 0.1 µM, state which is the major metabolic pathway out of Pathway 1 and Pathway 2, and justify your answer. iii) Using the information you have been given, calculate the rate of metabolism via Pathway 1 at a Compound X concentration of 0.1 μM. iv) Using the information you have been given, calculate the rate of metabolism due to Pathway 2 at a Compound X concentration of 40 μM
Compound X is metabolised by two distinct primary metabolic pathways. In vitro metabolism studies have found that Pathway 1 has a KM of 5 µM and a Vmax of 50 pmol/min/mg protein, whereas Pathway 2 has a KM of 20 μM and a Vmax 5 pmol/min/mg protein. i) At a Compound X concentration of 0.1 µM, calculate the intrinsic clearance values for (a) Pathway 1, (b) Pathway 2, and (c) overall metabolism by these two pathways ii) At a Compound X concentration of 0.1 µM, state which is the major metabolic pathway out of Pathway 1 and Pathway 2, and justify your answer. iii) Using the information you have been given, calculate the rate of metabolism via Pathway 1 at a Compound X concentration of 0.1 μM. iv) Using the information you have been given, calculate the rate of metabolism due to Pathway 2 at a Compound X concentration of 40 μM
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter27: Metabolic Integration And Organ Specialization
Section: Chapter Questions
Problem 4P
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