Compute for the appropriate amount (mL) of the compostions below of CTAB precitpitation solution used for DNA extraction. a. 1% w/v CTAB b. 10 mM EDTA c. 50 mM Tris-Cl
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Compute for the appropriate amount (mL) of the compostions below of CTAB precitpitation solution used for DNA extraction.
a. 1% w/v CTAB
b. 10 mM EDTA
c. 50 mM Tris-Cl
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Solved in 3 steps
- In HPLC, why is purging being done before analysis of samples. Outline the procedure on how purging is performed.For each measurement in this list indicate whether it must be made precisely (P) or approximately (A). Mass of EDTA Final Volume of EDTA solution Volume placed in polyethylene bottle Final volume of unknown solution Volume of HCl used to make 0.6 M solution Volume of methyl red used Volume of sample titrated Volume of EDTA used to reach end point Volume of polyvinyl alcohol usedCalculate the amount of phycocyanin in Sample 1 in mg where A620=0.211 and A650=0.086, taking into account the dilution factor as per question 6 (100ul), and the total volume of extract as per question 4 (140ml) . Note your answer to 2 decimal places
- Pick all that are TRUE regarding analysis of quaternary structures of proteins using polyacrylamide electrophoresis:I. The added β-mercaptoethanol disrupts S--S bonds bridging the polypeptide chains causing the appearance of higher Rf bands compared to the native protein run. II. Heating up any protein before subjecting to SDS-PAGE will always result in the formation of more than one band.III. A good asymmetrical gel layout would be : (Lane 1) MW ladder, (2) native protein, (3) protein + β-ME, (4) protein + HCL, (5) protein + β-ME + HCl.IV. Formation of a single band in the protein + β-ME + HCl run, whose Rf is lower than the native run, could be indicative that the protein is a homodimer.A. I onlyB. I and IIC. II and IIVD. None is true.pls explainIn HPLC, why is purging or initialization being done before analysis of samples, explain.Seperation of Amino Acids by thin layer chromotography Lab. Please answer #12 and #13 as I am very confused. Thank you for your help!
- Silica gel is very polar stationary phase. Therefore, more polar molecule sticks to silica gel on the TLC plate and has higherRfvalue. True Falsewhat is electrical double layer ? how it is formed ? how many types of micelles ate there give their application. asap fast or else skipCompute for the appropriate amount (mL_) of the compositions of a 10 mL 2x CTAB buffer for DNA extraction. a. 2% w/v CTAB b. 1.4 M sodium chloride c. 100 mM Tris-Cl d. 20 mM EDTA e. 2% polyvinylpyrrolidone MW 40 000
- Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.2% agarose gel, where the percentage is determined as mass/vol. How many grams of agarose must she add to 200 mL of buffer in order to arrive at the correct percentage?Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.233 and A650=0.061, using a dilution factor of 100, and a total volume of extract as 45 mL. Note your answer to 2 decimal places.Please give me explanation (1 to 2 pages) about: What is PVDF membrane? What are the advantages of PVDF membrane What is Hydrophobic and Hydrophilic PVDF Membranes What are the application of PVDF membrane?