Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.2% agarose gel, where the percentage is determined as mass/vol. How many grams of agarose must she add to 200 mL of buffer in order to arrive at the correct percentage?

Q: Agarose gel electrophoresis is relatively simple and straight forward to perform. Agarose is a…

A: two monomer units present in agarose are:

Q: Sequencing. Arrange the following procedures according to the CORRECT manner of preparing products.…

A: Turpuntine emulsion preparation AB AC  A B C E

Q: Explain the process of Designing a Multistep Synthesis

A: Chemical synthesis refers to artificial means to carry out reactions in the lab using suitable…

Q: does melting point determination remove impurites in crude protein?

A: Concept- Melting point is the temperature at which solid will convert into liquid. Because it is a…

Q: Calculate the following DNA concentrations and determine the purity of the samples: DNA Dilution…

A: Formula used :-  Concentration of double stranded DNA = 50 µg/mL × OD260 × dilution factor.

Q: Briefly explain how anodic stripping analysis is able to be used for quantifying DNA

A: Cathodic or anodic stripping voltammetry have been used for a highly sensitive determination of…

Q: Fig. 1 shows the possible effect of pH on recovery and impurity removal at the selection of a…

A: The Y-axis of the graph represents the percent recovery of the impurity, and the X-axis of the graph…

Q: Show calculations for making 1X TAE gel running buffer and 1.25% agarose gel for your experiment.

A: Buffer is a solution that tends to maintain pH of the solution when acid or base is added to it.…

Q: Ten grams of hamburger (from a container of hamburger) were added to 90 mL of sterile buffer. This…

A: When a solute-solvent system is diluted by adding more solvent the amount of the solute in the…

Q: In iodometry, a student forgot to add H2SO4 before the addition of KI.   Is there a positive…

A: In iodometry, iodine is liberated by adding sulphuric acid to the solution of iodized salt.

Q: What are the different technical considerations in performing the serum protein electrophoresis…

A: Answer - The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to…

Q: 5. A scientist has a mixture of two peptides, A and B, and wants to separate them using ion exchange…

A: (a)   The resin is positively charged, i.e., anion-exchange resin.   So, we need a buffer where both…

Q: A sample containing histidine (pKas 1.8, 6.0, 9.3), lysine (pKas 2.2, 9.1, 10.5), and leucine (pKas…

A: Because a protein's net charge is pH-dependent, the sample may be eluted from IEX medium by varying…

Q: Use the following chromatogram of standard amino acids: 24 16 19 21 12 15 6,6 9 10 23 13 14 17 18 10…

A: In chromatography, a higher retention time is linked to a higher affinity for the stationary phase…

Q: A 25 mL liquid bleach sample is diluted with water to 1000 mL in a bubble gel. 25 mL of the diluted…

A: The balanced chemical reactions involved is as follows: 2 I- + OCl- + H2O = I2 + Cl- + 2 OH- 2…

Q: What is the difference between Mohr and serological pipette?

A: Difference between Mohr and serological pipette is given below.

Q: Define, explain and elaborate

A: The heat of reaction, the amount of heat that must be added or removed during a chemical reaction in…

Q: In what order would histidine, serine, aspartate, and valine be eluted with a buffer of pH 4 from a…

A: The order in which the following would the amino acids will be eluted with the buffer of pH 4 from a…

Q: Q23: Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She…

A: Given, we have 1.1% agarose gel which is mass/volume percentage  And the total volume of buffer is…

Q: 25 mg / 100 mL.  A.Calculate the percent concentration of TPA in the solution.…

A: A) 0.025% w/v TPA B) 0.025 ml

Q: Explain why the LC-MS-MS method is superior to the peptide mass mapping method.

A: Peptide mass fingerprinting (PMF) is an analytical technique for protein identification in which the…

Q: What are the basic medium, acid medium, and zwitterion formulas of phenylalanine?

A: Phenyl alanine is an amino acid. Amino acid exist in three form depending upon the pH of solution.…

Q: You compare the proteins from blood samples from 2 siblings; one with blond hair and the other with…

A:

Q: Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out)…

A: Ammonium sulfate precipitation is one of the most commonly used methods for large and laboratory…

Q: A protein with a pI of 7.5 will be purified using ion-exchange chromatography.  Which ion exchange…

A: b)  A resin with sulfonic acid group is the correct option.

Q: In Kjeldahi analysis, a flour sample weighing 0.9857 was digested in concentrated H,So, for 45 min.…

A: Given the volume of 0.1011 N H2SO4 treated with NH3 extracted from the sample = 50.00 mL * (1L/1000…

Q: To establish 50% ammonium sulfate saturated solution, 314 g/l of salts should be added. Calculate…

A: It is given in question that solubility of salt is 314 g/L.  This means 314 g of salt is soluble in…

Q: ttering technique. If the formulation of the subject sample has included surfactants or salt…

A: The size of small particles in solution or in suspension is determine by the light scattering…

Q: Calculate the pH at each of the following points for the titration of 100 mL of 0. 6 M dl-Histidine…

A: A triprotic acid is one that can dissociate to furnish three protons by three equilibrium equations.…

Q: what are advangtage and disadvantage of using an Atomic Force Microscopy and explain each

A: Atomic force microscopy is a technique by which we study the different type of surfaces like…

Q: What are the types of MSDS forms.

A: MSDS forms means  Material Safety Data Sheet . It contains information about the various types of…

Q: which of the following chromatographic methods is best used for separating amino acids?

A: Chromatography is the technique that is used for the separation of different components from the…

Q: An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl-sepharose…

A: Ion exchange chromatography is an analytical technique for separation of proteins on the basis of…

Q: While purifying a new protein using a dextran Sephadex, the consistency of the column material…

A: As per the bartleby expert guidelines, I am allowed to answer one question at a time .  Please…

Q: justify the ingridients of benadryl cream

A: The Benadryl cream is actually an itch stopping cream. It is used to relieve itching and pain…

Q: What are analytes and how can they be used for biochemical recognition?

A: The unknown biological compounds or elements, such enzyme substrate, complementary DNA, and antigen,…

Q: 3. Isoelectric focusing is a separation technique that takes advantage of the differences in…

A: Isoelectric focusing: It separates the different amino acids based on their differences in their pI…

Q: If prior to the analysis, the protein X needs to be solubilised in 250 mM acetate buffer, pH 4.0 to…

A:

Q: Calcylafe the contentrafion , epuilibrivm congtant

A:

Q: What are annulenes ?

A: The compounds containing carbon and hydrogen only are termed as hydrocarbons. Hydrocarbons are…

Q: When analyzing a PTH (phenylthiohydratoin) derivative of the amino acid isoleucine, resulting from…

A: Reversed-Phase Chromatography: It is a liquid chromatography technique that involves the separation…

Q: Report the equation of the calibration curve and proof of linearity (R2 value).

A: We firstly need to determine the concentration of protein in each sample. Concentration of protein…

Q: Polyacrylamide gels (PAGS) are made by mixing acrylamide with bisacrylamide as a crosslinker. The…

A: Single-percentage gels are used to separate bands that are close in molecular weight. Optimum…

Q: How does the shape of a titration curve confirm the fact that the pH region of greatest buffering…

A: As there are three question are present I answered only one. you can repost your remaining two…

Q: (b) Protein concentration can also be determined by UV spectrophotometry. (i) Describe the principle…

A: Answer. UV spectrophotometry or UV-Visible spectroscopy  is an analytical technique used to identify…

Q: P UTF-8 Chapter 12 Solutions-20 x+…

A: Given-> Initial Volume = 2.0 L Initial molarity = 6.0 M Final volume = 6.0 L On dilution moles…

Q: In SDS page, the elute from the column first. proteins will move through the gel fastest and first,…

A: SDS page and size exclusion chromatography are methods to separate proteins . In SDS page, charged…

Q: Name four factors (bonds or other forces) that contribute to stabilizing the native structure of a…

A:

Q: Studies on cAMP actions in cultured cells usually involve adding to the cell culture not cAMP, but…

A: Cyclic adenosine monophosphate (CAMP) is a secondary messenger molecule that plays an important role…

Polymerization Kinetics

Polymerization is a chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that diverge in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes. Polymerization is generally classified into step-growth and chain polymerization. Polymers are categorized into linear polymer and cross-linked polymers that are produced either by condensation or additional polymerization.