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- A 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3– to oxidize ascorbic acid to dehydroascorbic acid. After the oxidation was complete, excess I3– was back- titrated with 13.96 mL of 0.07125 M Na2S2O3 to starch indicator endpoint. What is the concentration of ascorbic acid (MM = 176.12) in the orange juice? Report the concentration ascorbic acid in mg/100mL. C6H8O6 + I3– → C6H6O6 + 3I- + 2H+ I3– + 2S2O32- → S4O62- + 3I2 A. 82.51 % B. 42.89 % C. 27.34 % D. 40.85 %In the preparation of a standard stock solution for protein analysis, 50 mg of bovine serum albumin (BSA) was dissolved in deionized distilled water to a final volume of 10 mL. What is the weight of BSA in 0.60 mL stock solution?A 0.9000-g of KIO3 is dissolved in 500-mL distilled water. A 50.0-mL aliquot portion was run down into an Erlenmeyer flask and 2-g of KI and 2-mL of 6M HCl were added to it. Directly after, the solution was titrated with a 24.80-mL Na2S2O3 solution to a faint yellow solution. Starch TS was added to the resulting solution and the titration with the Na2S2O3 solution continued wherein an additional 1.33-mL was required to bring the blue color of the solution to disappear completely. MW: KIO3 = 214.0 g/mol ; Na2S2O3 = 158.1 g/mol Compute the N of the Na2S2O3 solution. None of the choices 0.09657 N 0.1075 N 0.1017 N
- Following the monograph procedure, a 724-mg of aspirin (MW+180 g/mol) dissolved in 18.5 ml of cold neutralized alcohol. This solution was then initially titrated with 0.101 N sodium hydroxide solution, then later neutralized with 0.104 sulfuric acid. 1. What is the milliequivalent weight consumed by the acidic titrant? a. 1.5392 g-meq b. 2.0907 g-meq c. 1.4948 g-meq d. 2.1528 g-meq 2. What is the milliequivalent weight consumed by the basic titrant? a. 5.8656 g-meq b. 1.5392 g-meq c. 5.6964 g-meq d. 1.4948 g-meq 3. What is the difference of milliequivalent weight consumed in the reaction? a. -4.1572 g-meq b. 4.3708 g-meq c. 0.5515 g-meq d. 4.1572 g-meq1.What is the significance when KD is either a small or large value? 2. Methyl alcohol and ethyl alcohol are poor extraction solvents. Why?3. What is the major disadvantage of using ether as an extracting solvent?19- A 25 mL liquid bleach sample is diluted with water to 1000 mL in a bubble gel. 25 mL of the diluted sample is transferred to the flask with a pipette, excess KI is added to it, and thus OCl- ion turns into Cl- ion and I2 is formed. The released iodine is titrated with 0.09892 M Na2S2O3 using the starch reagent, and 8.96 mL is required for the endpoint. Find the % NaOCl (w/v; w/v) in the bleach sample. (Na=23 g/mol, O= 16 g/mol, Cl = 35,44 g/mol)
- What is the purpose of adding sodium carbonate in the mixture of water and water- soluble components obtained from the solid-liquid extraction step of isolation of alkaloids from tea leaves? A.Increases the solubility of alkaloids in water thus ensuring maximum yield B.deprotonates tannins so that they will remain in the aqueous layer during liquid-liquid extraction C.'hydrolyses cellulose thus separating it along with other water-soluble components D.protonates the alkaloid to make the solution acidicThe ethyl acetate concentration in an alcoholic solution was determined by diluting a 10.00-mL sample to 100.00 mL. A 20.00-mL portion of the diluted solution was refluxed with 40.00 mL of 0.04672 M KOH:CH3COOC2H5 + OH- → CH3COO- + C2H5OHAfter cooling, the excess OH2 was back-titrated with 3.41 mL of 0.05042 M H2SO4. Calculate theamount of ethyl acetate (88.11 g/mol) in the original sample in gramsChemistry 1. Answer the following question with respect to Fatty Acid Methyl Esters (FAMEs) by Gas Chromatography for Determination of Fatty Acid Profiles in Foods. a) With the BF3 method for FAME preparation, glycerides and phospholipids are saponified by ________, and and fatty acids are esterified by _________. b) What are the functions of hexane and saturated NaCl? c) Summarize the safety concerns of BF3 and what we can do to minimize the hazard to yourself during the experimentexperiment.
- 1. Based on your knowledge on pKa, pH and ionisation describe a method whereby you can separate a solution containing: Sodium bicarbonate and ascorbic acid. 2. Briefly discuss why water is the liquid of choice in (1) car cooling systems and (2) in houses’ central heating systems. 3. Explain how to prepare standard solutions (5 ml each) of NaCl with the following concentrations: 10, 20, 30, 50, 70 ppm. The stock solution has a concentration of 0.05% w/v.Explain the purpose of the (1) Dowex-50X2-100 ion exchange resin and (2) K2CO3 in the Fischer Esterfication experiment ?A 0.9134-g of KIO3 is dissolved in 500-mL distilled water. A 50.0-mL aliquot portion was run down into an Erlenmeyer flask and 2-g of KI and 2-mL of 6M HCl were added to it. Directly after, the solution was titrated with a 24.47-mL Na2S2O3 solution to a faint yellow solution. Starch TS was added to the resulting solution and the titration with the Na2S2O3 solution continued wherein an additional 1.33-mL was required to bring the blue color of the solution to disappear completely. MW: KIO3 = 214.0 g/mol ; Na2S2O3 = 158.1 g/mol Compute the M of the Na2S2O3 solution. 0.1047 M None of the choices 0.1107 M 0.09926 M