Consider a batch culture of sphere-shaped bacteria in a growth medium or broth, in which the mean cell diameter of the bacteria is 2.0 μm (micron).  Show all your calculations and assumptions in answering the questions below: What is the volume of one of these sphere-shaped cells, expressed in liters? If there are 3 x 10^12 cells in one liter of this culture broth, what percentage of the volume is occupied by bacteria and what percentage is occupied by the cell culture broth (water only)? If the cells contain 80% water, what is the dry cell concentration, expressed as “grams dry weight/Liter of broth”?   You should assume that the density of the cells dry mass is approximately 1.0 gr/cm3

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Consider a batch culture of sphere-shaped bacteria in a growth medium or broth, in which the mean cell diameter of the bacteria is 2.0 μm (micron).  Show all your calculations and assumptions in answering the questions below:

  1. What is the volume of one of these sphere-shaped cells, expressed in liters?
  2. If there are 3 x 10^12 cells in one liter of this culture broth, what percentage of the volume is occupied by bacteria and what percentage is occupied by the cell culture broth (water only)?
  3. If the cells contain 80% water, what is the dry cell concentration, expressed as “grams dry weight/Liter of broth”?   You should assume that the density of the cells dry mass is approximately 1.0 gr/cm3
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