in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following: *Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range *Type of Centrifugation

in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following: Main type of centrifuge Subtype centrifuge * Speed range Operational temperature range Type of Centrifugation

Name: in isolating ribosomes from a yield sample, describe the ideal type of
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range*Type of Centrifugatio

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 3CS

See similar textbooks

Similar questions

in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Type of Centrifugation* Type of fraction *Give 2 advantages of using this type of centrifuge.*Give 2 disadvantages of using this type of centrifuge.
What other method could be used to purify lysozyme from white using its unusual pl?
Why differential centrifugation have poor yield, poor resolution and the fact that preparations obtained are never pure?
Using a schematic diagram, summarize the following steps in preparing competent cells for transformation: Inoculate a single colony of E. coli into 5 ml LB broth and incubate overnight at 37°C with moderate shaking (250 rpm). Add 200 μl of the culture into 50 ml LB broth and incubate overnight at 37°C with moderate shaking (250 rpm) to an OD600 = 1.3 to 1.5. Aliquot culture into five 15-ml pre-chilled, conical tubes. Leave tube on ice 5 to 10 min. Centrifuge cells 7 min at 1,600 × g (3,000 rpm), 4°C. Pour off supernatant and resuspend each pellet in 10 ml ice-cold CaCl2 solution (50 mM CaCl2), perform resuspension very gently, and keep on ice. Centrifuge cells 5 min at 1,100 × g (2,500 rpm), 4°C. Discard supernatant and resuspend each pellet in 10 ml ice-cold CaCl2 solution. Keep resuspended on ice for 30 min. Centrifuge cells 5 min at 1,100 × g, 4°C. Discard supernatant and resuspend each pellet in 10 ml ice-cold CaCl2 solution. Dispense cells (250 μl) into pre-chilled, sterile…
How can agarose gel electrophoresis process be imporved? What are ways to do it?
What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for? What tool is used to inoculate this medium?
Explain how you would identify the separation of lysozyme from a mixed sample using electrophoresis.
Why is GAPDH not be a suitable loading control for nuclear extracts?
You are tasked with cultivating the following microorganisms:A recombinant strain of E. coliA wild strain of Streptomyces clavuligerus• Suggest a storage method for each microorganism and justify your choice. You have at your disposal a refrigerator and an ultrafreezer, in addition to materials and conditions for handling microorganisms.• Propose a culture medium for each (choose a complex medium for one organism and a synthetic medium for the other)As a complement of nutrients, they must be indicated on the following scale: +++ : above 5 g/L++ : from 1 to 5 g/L+ : below 1 g/L• Describe the procedure for preparing and sterilizing the components of the culture media for a volume of 5.0 L• Make a bench process selection scheme, up to the step of selecting a benchtop reactor 5 L.
In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?
What are the advantages and disadvantages of differential centrifugation?
In bacterial transformation, What is the purpose of ampicillin and arabinose in the growth media?