What would the activity of a lysozyme sample be if it was able to change the absorbance of a cell wall substrate sample from 0.815 to 0.610 in 45 seconds?
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What would the activity of a lysozyme sample be if it was able to change the absorbance of a cell wall substrate sample from 0.815 to 0.610 in 45 seconds?
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- using the cell potential table found in the Reference Material, why do you get holes in your aluminum foil if you use it to cover lasagna in a steel pan? Hint: Steel is made up of mostly iron and lasagna serves as an acidic medium.Bacteria are typically grown at a temperature of 37oC. What happens to the membrane lipid composition if the temperature is increased to 42oC?Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.
- We have 10 mL of cells to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 20 µM. How much of a 30 mM stock solution of H2O2 would we add to our cells? If you could please help me understand the steps of this problem!What is meant by the statement “orthogonal analysis methods should be used for characterization of impurities”? Why do bacterial and plant cells require partial destruction prior to osmotic lysis? How can the partial destruction be achieved?If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?
- Adenine phosphoribosyltransferase converts adenine to AMP. If you were to determine whether a similar reaction converts adenine directly to dAMP, what metabolite would you need to find in cells at appreciable concentrations?During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why is the log value of the cell concentration used?You have 12 mL of cells you need to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 50 uM. How much of a 30 mM stock solution of H2O2 would you add to your cells?