Consider the complete oxidation of one mole of simple TAG containing lignoceric acid residues (24:0). 1. For one mole of the fatty acid residue, determine the following: a. ATP yield obtained from the oxidation of acetyl CoA entering the TCA cycle and ETC b. ATP yield obtained from NADH coming from the complete B-oxidation of the fatty acid residue c. ATP yield obtained from FADH₂ coming from the complete ß-oxidation of the fatty acid residue d. net ATP yield of ATP in the complete oxidation of the fatty acid residue 2. What is the net ATP yield for the complete oxidation of all the fatty acid residues of the simple TAG? (Note: glycerol backbone is not included)
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- Using the ActiveModel for enoyl-CoA dehydratase, give an example of a case in which conserved residues in slightly different positions can change the catalytic rate of reaction.Extending the Mechanism of Methylmalonyl-CoA Mutase to Similar Reactions Based on the mechanism for the methylmalonyl-CoA mutase (see problem 14), write reasonable mechanisms for the following reactions shown.Figure 27.3 illustrates the response of R (ATP-regenerating) and U (ATP-utilizing) enzymes to energy charge. a. Would hexokinase be an R enzyme or a U enzyme? Would glutamine: PRPP amidotransferase, the second enzyme in purine biosynthesis, be an R enzyme or a U enzyme? b. If energy charge = 0.5: Is the activity of hexokinase high or low? Is ribose-5-P pyrophosphokinase activity high or low? c. If energy charge = 0.95: Is the activity of hexokinase high or low? Is ribose-5-P pyrophosphokinase activity high or low?
- Understanding the Mechanism of the HMC-CoA Synthase Reaction Write a suitable mechanism for the HMG-CoA synthase reaction. What is the chemistry that drives this condensation reaction?Examine the ActiveModel for alcohol dehydrogenase and describe the structure and function of the catalytic zinc center.Consider the complete oxidation of one mole of simple TAG containing behenic acid residues (22:0). I. For one mole of the fatty acid residue, determine the following: a. ATP yield obtained from the oxidation of acetyl CoA entering the TCA cycle and ETC b. ATP yield obtained from NADH coming from the complete β-oxidation of the fatty acid residue c. ATP yield obtained from FADH2 coming from the complete β-oxidation of the fatty acid residue d. net ATP yield of ATP in the complete oxidation of the fatty acid residue II. What is the net ATP yield for the complete oxidation of all the fatty acid residues of the simple TAG? (Note: glycerol backbone is not included)
- Consider the complete oxidation of one mole of arachidic acid (20:0). ___________ a. How many rounds of the β-oxidation pathway will be involved? ___________ b. How many acetyl CoA will be produced? ___________ c. How many NADH will be produced from all the rounds of the β-oxidation pathway? ___________ d. How many ATP will eventually be produced from the complete oxidation of one mole of arachidoyl CoA?Consider the complete oxidation of one mole of simple TAG containing behenic acid residues (22:0). I. For one mole of the fatty acid residue, determine the following: a. What is the number of ATP yield obtained from the oxidation of acetyl CoA entering the TCA cycle and ETC b. What is the number of ATP yield obtained from NADH coming from the complete β-oxidation of the fatty acid residue c. What is the number of ATP yield obtained from FADH2 coming from the complete β-oxidation of the fatty acid residue d. What is the number of net ATP yield of ATP in the complete oxidation of the fatty acid residue II. What is the net ATP yield for the complete oxidation of all the fatty acid residues of the simple TAG? (Note: glycerol backbone is not included)Consider the complete oxidation of one mole of the fatty acyl CoA of capric acid (10:0). Determine the following: rounds of β-oxidation total no. of acetyl CoA produced total no. of NADH produced from all rounds of β-oxidation total no. of FADH2 produced from all rounds of β-oxidation total no. of NADH produced from the oxidation of all acetyl CoA in the TCA cycle total no. of FADH2 produced from the oxidation of all acetyl CoA in the TCA cycle total ATP yield
- Consider the complete oxidation of one mole of simple TAG containing behenic acid residues (22:0). I. For one mole of the fatty acid residue, determine the following: a. ATP yield obtained from the oxidation of acetyl CoA entering the TCA cycle and ETC b. ATP yield obtained from NADH coming from the complete β-oxidation of the fatty acid residue c. ATP yield obtained from FADH2 coming from the complete β-oxidation of the fatty acid residueConsidering the complete oxidation of an 18-C fatty acid. Give the answer for the following question.a. How many rounds of beta-oxidation is needed?b. How many NADH molecules are produced in beta-oxidation only?c. How many FADH2 molecules are produced in beta-oxidation only?d. How many Acetyl Co-A are produced?In the complete b-oxidation of stearic acid (18:0) How many moles of acetyl CoA are produced? How many times would the fatty acid pass through the fatty acid spiral for complete oxidation?