Correct answers already provided! Please don't just tell me the answers bc I know them already. Help me with my own question.I get everything else in this problem other than the third option: Introduce the mutant human HD allele as a transgene into the mouse genome with transgene integration anywhere in the mouse genome.Why is the first question (left) okay with introducing mutant human HD allele and the second question (right) is not? I heard that introducing allele without using CRISPR-Cas9 is very rare and difficult. If so, how does it work in the first problem (Hungtinton's chorea)? You would like to create a mouse model for spinal muscular atrophy, a recessive human disease caused by a loss of functionYou would like to create a mouse model of the human disease Huntington's chorea, a dominant disease caused by a gain of mutation in the SMN1 gene. Which of the following strategies would be suitable? Select all that applyfunction mutation in the human HD gene. Which of the following strategies would be suitable? Select all that applyUse CRISPR-Cas9 technology to cut the mouse HD gene; supply the mutant human HD allele as a repair templateand select for transgenics where the the mutant human HD replaced the mouse HD geneUse CRISPR-Cas9 to introduce frameshift mutations in the mouse HD gene; breed the resulting mutant mice togenerate homozygous mutantsIntroduce the mutant human HD allele as a transgene into the mouse genome with transgene integrationanywhere in the mouse genomeIntroduce the wild type human HD allele as a transgene into mouse genome; select transgenics where humanHD replaced the wild type mouse HD gene via homologous recombinationUse CRISPR-Cas9 to introduce frameshift mutations in the mouse SMN1 gene; breed the resulting mutant mice togenerate homozygous mutantsUse CRISPR-Cas9 technology to cut the mouse SMN1 gene; supply a mutant human SMN1 allele as a repairtemplate and select for transgenics where the the human allele replaced the mouse allele of SMN1; breed theresulting mutant mice to generate homozygous mutantsIntroduce a mutant human SMN1 allele as a transgene into mice, with transgene integration anywhere in themouse genome; breed the resulting transgenics to generate homozygotesIntroduce a wild type (functional) human SMN1 allele as a transgene into mice; select transgenics where thehuman gene replaced the wild type mouse SMN1 gene via homologous recombination

In the realm of genetic research, producing accurate animal models of human diseases is essential…

Correct answers already provided! Please don't just tell me the answers bc I know them already. Help me with my own question. I get everything else in this problem other than the third option: Introduce the mutant human HD allele as a transgene into the mouse genome with transgene integration anywhere in the mouse genome. Why is the first question (left) okay with introducing mutant human HD allele and the second question (right) is not? I heard that introducing allele without using CRISPR-Cas9 is very rare and difficult. If so, how does it work in the first problem (Hungtinton's chorea)?

Correct answers already provided! Please don't just tell me the answers bc I know them already. Help me with my own question. I get everything else in this problem other than the third option: Introduce the mutant human HD allele as a transgene into the mouse genome with transgene integration anywhere in the mouse genome. Why is the first question (left) okay with introducing mutant human HD allele and the second question (right) is not? I heard that introducing allele without using CRISPR-Cas9 is very rare and difficult. If so, how does it work in the first problem (Hungtinton's chorea)?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 19QP

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Similar questions

You want to identify and compare the gene expression profile of several genes in a disease. Will you be able achieve this using Q-PCR technique? Can you identify a potential mutation (the nucleotide change) within a gene sequence using conventional PCR? If not, then what approach you should take?
You are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture of E. coli. After you run the experiment, you decide to use gel electrophoresis to genotype the different bacterial cultures to determine if the gene editing was successful. How could your electrophoresis results confirm that the PCR was successful? And how could your electrophoresis results confirm that you successfully extracted genomic DNA from your bacterial samples?
In conventional PCR applications, a heat-resistant polymerase is used. Why? You want to identify and compare the gene expression profile of several genes in a disease. Will you be able achieve this using Q-PCR technique? Can you identify a potential mutation (the nucleotide change) within a gene sequence using conventional PCR? If not, then what approach you should take?
If you had the ability to do gene editing with ONE gene for the betterment of human kind, which one would you choose, and why? Assume you could either change an abnormal allele associated with a disease, such as the cystin gene associated with Cystic Fibrosis to its normal wild type, or add a pre-existing human allele to a genome.
Attached is a cartoon model of the CRISPR system, with 3 critical components labeled. A. What is the identity of each component? B. What is the function of component a? C. What is the function of component c?
Which of the following statements is not true about the guide RNA (see this interactive demonstration for help with this question: https://www.biointeractive.org/classroom-resources/crispr-cas9-mechanism-applications) Group of answer choices it contains a sequence of 20 nucleotides that matches a specific sequence in a cell’s DNA When the guide RNA is combined with Cas9, it will guide Cas9 to the target sequence its target sequence can be almost any sequence as long as it occurs near a PAM motif it is a nuclease, a type of enzyme that cleaves DNA
Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer choices A single guide RNA (sgRNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence A single guide RNA (sgRNA) recognizes a genomic region followed by a long sequence palindrome repeat A single guide DNA (sgDNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence PAM sequences induce single stranded breaks that are then repaired by the CAS-9 enzyme
CRISPR techniques allow scientists to modify specific genes while sparing all others, thus clarifying the association between a given gene and its consequence to the organism. If this technology can change the future of Medicine, what specific benefits CRISPR can bring to genetic testing or analysis? How can CRISPR help to enhance gene therapy or treatment of genetic diseases?
When doing a lab that involves Extraction of Genomic DNA from adult Drosophila melanogaster. - What are the controls you should use and why use these? What is special about the genomic DNA? What do you expect to find (using these genomic DNA samples)? please help!
Let’s suppose you are in charge of organizing and publicizing a databasefor the mouse genome. Make a list of innovative strategies you wouldinitiate to make the mouse genome database useful and effective.
Suppose a 10-year old patient has come to your office with a very rare disease. One so rare that only 100 people for the past 100 years have been diagnosed and nobody knows the gene or genes that are mutated in this disease. Describe the gene sequencing toold you would use to identify the mutated gene or genes in this hypothetical disease.
Some people have argued that editing the genomes of human embryos is ethically defensible as long as the embryos are not allowed to develop past an early stage and thus will never result in the birth of genetically modified humans. Others have argued that no genome editing should be carried out on human embryos. Present arguments for and against using genome editing on human embryos.