David is in dilemma to choose a suitable chromatographic detector for gas chromatography analysis. Name a suitable detector to determine pesticide content in vegetables. List 2 characteristics of a good detector.
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Q: What factor allows molecules to be separated by chromatography?
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Q: Which of the following are necessary steps in performing paper chromatography? I. Saturate the…
A: In paper chromatography the necessary conditions are 1. The chamber should be saturated with the…
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A: Given, Width of half peak height , w12= 12 mm retention time , tr = 650 mm
Q: what is chromatography? Explain different types of chromatography and explain how to seperate…
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Q: Which of the following statements about a gas chromatogram is correct? O Tailing indicates that the…
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Q: Question 2 Retention time is the time spent by analyte in both mobile and stationary phases. True…
A: Kindly get the answer given below.
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Q: igh-performance liquid chromatography would never be applied in drug analysis This statement is…
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Q: 2. Different marker inks were analyzed using a simple paper chromatography experiment. Two marker…
A: To calculate the retention factor of each spot from A to E.
Q: Which of the following statements about the mobile phase in Supercritical fluid chromatography (SFC)…
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Q: size exclusion chromatography
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Q: Chromatography
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Q: High performance liquid chromatography can separate eluates even if they are not present in the UV…
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Q: 7] Which of the following are necessary steps in performing paper chromatography? I. Saturate…
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Q: Suggest two possible applications for paper chromatography other than the separation of dyes or the…
A: Paper chromatography is a technique used for separation of components of a mixture. In paper…
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A: Gas chromatography can be performed only in columns.
Q: Explain the following terms used in chromatography i) Dead time ii) Number of theoretical plates
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Q: Choose the separation techniques that rely on differences in boiling points. A. Simple distillation…
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Q: i) Put the steps of a column chromatography analysis in a correct order. a. Elute the column with…
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: A mixture of species of A, B, and C is injected into a liquid chromatographic column with length of…
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Q: State 2 properties of detectors used in chromatography?
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Q: Explain the following Chromatography (for drug analysis) Thin-layer chromatography (TLC) Gas…
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Q: Peak area is used for quantitative analysis in gas-liquid chromatography. True False
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Q: Retention time is the time taken for the mobile phase to reach the detector. True False
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Q: Which statement(s) are correct for gas chromatography? A) the components in a mixture can be…
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- Which of the following are necessary steps in performing paper chromatography? I. Saturate the chamber with the vapor of the mobile phase II. Allow the chromatogram to touch the sides of the beaker III. Place the spots at the same height as the solvents IV. Spot the samples 2 cm apart from each other a. I and IIIb. I and IVc. I, III and IVd. II, III and IVPlease answer Questions 5-3, 5-4, and 5-5 (GC Gas Chromatography) 5-2. A sample mixture of ethyl benzoate (bp 212 Celsius) and dodecane (bp 216.2 Celsius) is injected on two GC columns.Column A has DC710 silicone oil as the stationary phase, and column B uses polyethylene glycol as the stationaryphase. Which substance would be certain to elute first from column A and would the same material be expected toelute first from column B? Which column, A or B, would be expected to give the better separation of these two substances? 5-3. Question 5-2 refers to separating a mixture of two high boiling liquids by gas chromatography. These materials havesimilar boiling points. List several GC variables and conditions that would make it easier to separate thesesubstances by gas chromatography. 5-4. Capillary GC columns have better resolution than packed columns even though the enormous surface area provided by the packing material is absent in capillary columns. Why? 5-5. Preparative GC requires…Which type of chromatography is paper chromatography? a.Column normal phase chromatography b.Planar reverse phase chromatography c.Column reverse phase chromatography d.Planar normal phase chromatography
- Explain the following Chromatography (for drug analysis) Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC)Which of the following chromatographic methods cannot use a mixture of liquid solvents (eluents) during the separation process? Thin layer chromatography High pressure liquid chromatography Column chromatography Gas chromatographyState 2 properties of detectors used in chromatography?
- Each of the following could lead to poor liquid chromatographic separation. Explain how or why each one would decrease the separation of two molecules. collection of large elution fractions very slow flow rate of mobile phase too weak of an adsorbent1. For the following chromatography state what the mobile phase is, stationary phase is, and what would elute first for each of the chromatography type... HPLC Gas Chromatography Ion Exchange Chromatography Size Exclusion Chromatography (SEC) Affinity Chromatography Reverse Phase Chromatography Normal Phase ChromatographyWhich statements are true for molecular exclusion chromatography?I Molecular exclusion chromatography separates on the basis of size.II Small molecules that freely pass through the stationary phase, Kav = 0.III Small molecules can fit in the pores of the stationary phase and effectively pass through a larger volume, and elute last.IV The pores in the stationary phase are too small for large molecules to pass through, so large molecules elute last.V Molecular exclusion chromatography with a hydrophilic stationary phase and an aqueous solvent is called gel permeation chromatography. A. II and V B. I, II, and IV C. III, IV, and V D. No correct combinations given above E. I, III, and IV F. I, II, III, and V
- Explain how separation generally occurs in chromatography. What are the factors affecting the Rf value in a chromatographic analysis?2. a) What is a bonded phase in HPLC?b) Give an example of a bonded phase in reversed-phase liquid chromatography and normal phase liquid chromatography.c) Describe when you would choose to use a reversed-phase column or a normal-phase column for an analysis of a mixture.assignment 3. an exhibit shows a peak at 2.4 minutes in a gas chromatogram. a sample of known heroin also has a retention time of 2.4 minutes using the same instrument under the same conditions. the fact that both the unknown and the known have the same retention time is indicative of their being the same substance, but it does not prove this relationship. discuss?