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- Describe a technique that will allow you to separate cells from media.Identify Four (4) possibilities in cell culture practices that able affect the quality and yieldof biomedical production in the industry. use computer typing to answer thisExplain the image. How does the results of electrophoresis must be read?
- Select a type of microscope and specimen preparation method to observe the following biological samples and explain the rationale for your choice - Determine if a surgical specimen is cancerous.outline a protocol of cell culture techniques from call thawing to incubating.Explain the basis for identification using DNA fingerprinting. – relate this to Microbiology not to human fingerprinting. Why does this technique work? Mention restriction enzymes and their function.
- briefly discuss why Natural environments differ significantly from the conditions that are created in a laboratory for thegrowth of micro-organisms.PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in.PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in. • Leave the petri dishes uncovered while the bacteria grow. , cover the petri dishes while the bacteria grow. Treat the bacterial culture with a disinfectant before spreading on the agar plate Wear personal protective equipment (gloves, mask, lab coat) while performing the procedure Wear…
- Match the following experimental methods with their specialty procedures and objectives. autoradiography use of green fluorescent protein (GFP) fractional centrifugation use of genetic mutants A. silencing genes means inhibition of the expression of a protein B. fluorescing GFP protein is fused with a protein tracked in the cell C. biochemical processes are visualized using radiolabeled materials D. tissue samples homogenized at varying centrifugal force leads to isolation of desired cellular fractionDescribe an experiment for testing to determine whether or not the bacteria have been successfully transformed.explain why the invention of solid culture media was of great importance to the development of microbiology as a science
