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What is the best streaking technique in a culture media? Explain
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- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?What is the difference between a mixed culture and a contaminated culture if they both contain more than one type of organism ?
- What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture can be stored.
- What is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?What is the purpose of adding sodium bicarbonate to a culture medium?Why do you think the continuous culture has higher chances of contamination than batch and fed-batch culture?