Define what is a minimal medium and the nature of its components. Three different strains of Escherichia coli, respectively A, B, and C have been inoculated in threedifferent media, namely I, II, and III, and the growth of each bacterial strain has been followed byspectrophotometry (Figure 1).Strain AStrain BStrain C4.545Medium I4.3.5252.53Minimal medium+ Glucose2.5251.5150.5as0510020030040050060010020010000s0060010020030000Time (min)Strain AसिStrain BetmStrain B4.5Medium II3.525253Minimal2.525medium1.5+ Glucose+ Arginine0.5asas1002003004005006001003000000sa0600100300400s00600meFime (mintStrain ATmeStreinBStreine4.sMedium III44.3.53.5253Minimal medium+ Glucose+ Lactose2.525251.5151.0.5050510020030040050060010020040010200S00S00630Time (min)Tine imin]Time (miniFigure 1. Growth curves of the three E. coli strains A, B and C in three media:I, minimal mediumcontaining glucose; II, minimal medium containing glucose and arginine; and III, minimalmedium containing glucose and lactose. The graphs represent the variations of optical densityrelated to time.Optical DensityOptical DensityOptical DensityOptical DeratyCatial DensityAag jeo

A culture medium can be defined as solid,liquid or semi solid medium which helps in support of the…

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Define what is a minimal medium and the nature of its components.

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter4: The Prokaryotes

Section: Chapter Questions

Problem 4UTM

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Concept explainers

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.

Question

Define what is a minimal medium and the nature of its components.

Three different strains of Escherichia coli, respectively A, B, and C have been inoculated in three
different media, namely I, II, and III, and the growth of each bacterial strain has been followed by
spectrophotometry (Figure 1).
Strain A
Strain B
Strain C
4.5
45
Medium I
4.
3.5
25
2.5
3
Minimal medium
+ Glucose
2.5
25
1.5
15
0.5
as
05
100
200
300
400
500
600
100
200
100
00
s00
600
100
200
300
00
Time (min)
Strain A
सि
Strain B
etm
Strain B
4.5
Medium II
3.5
25
25
3
Minimal
2.5
25
medium
1.5
+ Glucose
+ Arginine
0.5
as
as
100
200
300
400
500
600
100
300
00
00
sa0
600
100
300
400
s00
600
me
Fime (mint
Strain A
Tme
StreinB
Streine
4.s
Medium III
4
4.
3.5
3.5
25
3
Minimal medium
+ Glucose
+ Lactose
2.5
25
25
1.5
15
1.
0.5
05
05
100
200
300
400
500
600
100
200
400
10
200
S00
S00
630
Time (min)
Tine imin]
Time (mini
Figure 1. Growth curves of the three E. coli strains A, B and C in three media:I, minimal medium
containing glucose; II, minimal medium containing glucose and arginine; and III, minimal
medium containing glucose and lactose. The graphs represent the variations of optical density
related to time.
Optical Density
Optical Density
Optical Density
Optical Deraty
Catial Density
Aag jeo

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