Describe the findings of ribosome profiling that would show the presence of a regulatory mechanism acting at the translational initiation level.
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Describe the findings of ribosome profiling that would show the presence of a regulatory mechanism acting at the translational initiation level.
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- Please describe the four-step process of the elongation during protein translation in bacteria.Compare prokaryotic and eukaryotic translation initiation with respect todelivering an initiator tRNA to the ribosome and locating the initiation codon.Outline the steps in translation initiation observed in eukaryotes and compare them to those observed in bacteria and archaea
- Explain the steps of Bacterial Translation Initiation. 1. Formation of preinitiation complex. 2. Formation of 30S initiation complex. 3. Ribosome assembly.Compare bacterial and eukaryotic mRNAs, and explain the functional significance of their structural differences.Refer to the DNA sequence provided:3’ -TACTGAAGCGGCAGCCCCGCATGAGTAGACCTTACT-5’a. What is the mRNA transcript of the anticoding strand of the DNA model?
- List the 4 types of RNA degradation and describe each in 1 sentenceDefine the following terms: a. cap-binding complex b. 43S preinitiation complex c. poly(A)-binding protein d. 48S initiation complex e. glycosylation1. a)how is it possible for such drugs to selectively kill bacterial cells and not our own cells? b)Provide an example of post-translational regulation of protein activity and explain the advantage of regulating each protein/process at the post-translational level instead of the transcriptional level.
- List the critical steps in the ribosome profiling protocol and explain what this method is useful for when coupled with high-throughput sequencing.Outline the function of each of the subunits of E.coli RNA polymerase.How would a mutation in the poly(A)-binding protein gene affect translation? How would an electron micrograph of polyribosomes from such a mutant differ from the normal pattern?