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Describe the steps you would take to isolate, cultivate, and identify a
microbial pathogen from a urine sample.
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- What is the motivation behind the use of stab slant and surface inoculation?What things can be done during sample collection of a sick patient to help eliminate the culture of normal flora?Explain the activities associated with the isolation of Bacillus subtilis organsim from mixed culture to pure culture. Include the inoculum source, incubation temperature and other information.
- Explain the activities associated with the isolation of Bacillus megaterium organsim from mixed culture to pure culture. Include the inoculum source, incubation temperature and other information.What is the optimum growth temperature for most human pathogens? Explain.explain why you would expect to see similarities in the types of colonies on the fomite plates and the handwashing plates?
- What is the most commonly used fixative in stool samples and why? Why is staining of stool samples very important in identifying parasites? Why is direct fecal smear a routine procedure in the parasitology section?Describe the purpose of identifying unknown bacterial culture in medical field. Name the step by step experiments/procedures that microbiologists follow in order to identify microorganisms.Based on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *
- describe how the pure culture of mycobacterium ulcerans should be inoculated with the cultureWhy is glucose not the choice of carbohydrate for most differential media used in the identification of gram (-) enteric bacilli?Are the large numbers of microorganisms found in the mouth cause for concern? Explain. How can you determine whether a culture or subculture is pure? What kinds of clinical speciments may yield a mixed flora in bacterial cultures? Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?