What things can be done during sample collection of a sick patient to help eliminate the culture of normal flora?

Q: What gram-positive bacterium can give a positive presumptive test?

A: In microbiology, the presumptive test is used to find out the presence of gas-forming lactose…

Q: Why is it important to limit the quantity of cells used to prepare a smear?

A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…

Q: What is a mixed culture? A contaminated culture?

A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…

Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…

A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…

Q: What method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line…

A: An isolated colony represents a pure source of an organism from which a pure culture can be started.

Q: Why do you have to boil the agar solution before dispensing it into tubes?

A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…

Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…

A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…

Q: Why is the level of contamination measured as number of colonies rather than size of colonies?

A: Microbiological contamination refers to the presence of unwanted or accidental introduction of…

Q: What might happen if the Gram staining procedure is performed ona culture incubated for a little…

A: Gram staining is one of the staining methods used in laboratories to differentiate between two broad…

Q: How does the heaviness of a bacterial smear affect its microscopic analysis?

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: Why is staining of stool samples very important in identifying parasites?

A: In this question it is to describe that why is staining of stool samples very important in…

Q: Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?

A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…

Q: Describe the steps you would take to isolate, cultivate, and identify amicrobial pathogen from a…

A: The techniques used in the identification of microbes can be termed as the six "I's": inoculation,…

Q: shape

A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…

Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar…

A: Aseptic Technique describe some of the ways that a laboratory can deal with the constant threat of…

Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…

A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…

Q: I Why does't a negative stain colorize the cells in the smear?

A: Staining is a technique which is used for enhancement of contrast in samples mostly at the level of…

Q: a specimen from an infected wound has been submitted to the clinical laboratory. explain in detail…

A: Viruses, bacteria, fungus, protozoa, and helminths are the five types of disease-causing agents…

Q: Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?

A: Microbial taxonomy is a field of biological sciences that deals with the grouping of microorganisms…

Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…

A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…

Q: Give at least two other examples of samples that are best prepared using Smear Preparation…

A: Smear preparation technique: it is used to fix the samples onto the slides.

Q: What is the importance of using a test/control organism in describing the cultural characteristics…

A: The food materials on which the organism is developed is called culture medium and the development…

Q: Which step of the gram stain procedure is most critical?why?

A: Gram staining is one of the most common laboratories test to identify the different bacterial…

Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…

A: Preparation of culture media and inoculation are two of the very sensitive steps in any…

Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: Describe the steps of the Gram stain, and explain how it can be animportant diagnostic tool for…

A: Gram staining is a method used to differentiate and classify the bacterial species into two category…

Q: Explain the difference between Gram positive and Gram negative cell walls. Describe the procedure…

A: The bacteria are classified into Gram positive or Gram negative according to their nature of the…

Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?

A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…

Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…

A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…

Q: erial slide agglutination technique important in diagnostic procedure?

A: Immunoassays are bioanalytical ways that use the specificity of an antigen-antibody reaction to…

Q: Define the term gram staining.

A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…

Q: The main of objective of using 13-Line streaking method is to get:

A: Answer: 13-Line streaking method: It is the technique of obtaining pure bacterial culture by…

Q: Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that…

A: The differential stain is used to distinguish between different structures and cellular components.…

Q: if there are features of the gram stain that are not as expected what possible sources or error may…

A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: What is the best streaking technique in a culture media? Explain

A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…

Q: what is your initial assumption of the organism causing this disease

A: Bacteria are the prokaryotic microorganism and based on the structural cell walls, Gram stain…

Q: How is the Gram stain used clinically to help identify the cause of an infection?

A: Gram stain or sometimes "Gram staining", likewise called Gram's method, is a technique for staining…

Q: You want to isolate staphylococci from a clinical sample, as you suspect the patient nas an…

A: Staphylococcus aureus : It is gram positive , round shaped bacteria and is a usual member of…

Q: the chemicals used in the gram staining method?

A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…

Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…

A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…

Q: What advantage does the oil immersion objective have over the low power objective when doing…

A: Introduction:- Bacteria are prokaryotic and very small sized organism's . They can not be visualised…

Q: What would be the expected out come of a gram staining test If the bacteria is a pathogenic…

A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…

Q: Based on the results of this Gram stain and the ID matrix below, which of the following microbes…

A: Gram' s staining helps in bacterial identification and their further classification.  On the basis…

Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…

A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.

Q: Why should freshly collected samples be used for microscopic examination?

A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…

Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…

A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…

Q: Does the gram staining method still have a place in today's world of advanced diagnostic methods? If…

A: Gram staining is a type of staining which is used to differentiate bacteria on the basis of…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.