Determine the gross ATP from B-oxidation cycles, gross ATP from acetyl CoA produced, gross ATP from conversion of propionyl CoA (if applicable), total number of ATP deducted, and total net ATP. Please show all solutions and box the final answers.
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- Consider the fatty acids: (a) Arachidic acid (C20H40O2); molar mass = 312.5 g/mol) (b) Palmitoleic acid(C16H30O2); molar mass = 256.4 g/mol). i. How many cycles of β -oxidation are needed for complete oxidation?ii. How many molecules of acetyl CoA are formed from its complete catabolism?iii. Calculate the number of molecules (moles) of ATP formed (net) by the completecatabolism of each fatty acid (show your calculation).iv. Calculate number of moles of ATP formed per gram of each fatty acid metabolized.If, in addition to the constraints on glucose concentration listed previously, we have in the liver cell ATP concentration = 5 mM and ADP concentration = 5 mM , what is the theoretical concentration of glucose-6-phosphate at equilibrium at pH=7.4 and 37 ∘C ? Express your answer to three significant figures and include the appropriate units. Please type answer note write by hend.Consider fatty acids from the hydrolysis of the given TAG in a liver cell where amino acid and nucleotide biosynthesis is very active For each fatty acid given, determine the following. Gross ATP from b-oxidation cycles Gross ATP from acetyl CoA produced Gross ATP from conversion of propionyl CoA (if applicable) Total number of ATP deducted Total net ATP
- Complete the table below. Consider docosanoic acid (C21H43CO2H) Questions Solutions Answers a. Label the alpha and beta carbons. b. Draw the acetyl CoA derived from this fatty acid. c. How many acetyl CoA molecules are formed by complete B-oxidation? d. How many cycles of B-oxidation are needed for complete oxidation? e. How many molecules of ATP are formed from the complete catabolism of this fatty acid?Under aerobic conditions when glucose is limiting, with high ratios of NADH/NAD+ and ATP/ADP, as carbon-2 radiolabeled pyruvate is utilized for its carbon skeleton, which molecules would you expect to see significant radiolabeling in the liver? Select all that apply. **Please note some molecules contain more details, including not only molecule name, but location of the label. Pick the options that are accurate for the above situation. Glucose C-2 only Label is halved over many TCA cycles Oxaloacetate Glucose C-1 and C-6 Glucose C-2 and C-5 CO2 from TCA cycle shows some radiolabel Lactate C-2 for export Malate Pyruvate C-1Under aerobic conditions of high ratios of NADH/NAD+ and ATP/ADP, as pyruvate is utilized for its carbon skeleton, which molecules would you expect to see significant radiolabeling in the liver? Select all that apply. **Please note some molecules contain more details, including not only molecule name, but location of the label. Pick the options that are accurate for the above situation. 1. Glucose C2 & C5 2. Glucose C1 & C6 3. Glucose C2 only 4. Pyruvate C1 5. Lactate C2 for export 6.CO2 from TCA cycle shows some radiolabel 7. Label is halved over many TCA cycles 8. Oxaloacetate 9. Malate
- Looking at the structure of alpha linolenic acid, how many rounds of beta oxidation will occur to fully oxidize the fatty acid into acetyl CoA molecules? Please provide explanationsAfter determining the optimum pH, you are now tasked to determine the inhibitory effects of acarbose, a known anti-diabetic drug which acts an inhibitor of alpha-glucosidase, an intestinal enzyme that hydrolyzes starch to release glucose for absorption and energy usage of the body. You studied the rates of the enzyme at different glycogen concentrations with and without the presence of 0.10 mM acarbose. Below is the summary for this procedure. a) What is the Vmax, in arbitrary units, of the uninhibited reaction (without acarbose)? b) What is the KM , in mM, of the uninhibited reaction (without acarbose)? c) What is the type of inhibition?Myristoleic acid is a monounsaturated fatty acid found in small amounts in a variety of foods. Calculate the net ATP yield from the complete β-oxidation of myristoleic acid. The formula of myristoleic acid is shown below (it is assumed that the total ATP production is the same for both saturated and unsaturated fatty acids having the same carbon chain length). CH3-(CH2)3-CHCH-(CH2)7-COOH (Given: The oxidation of one NADH yields 2.5 ATP; the oxidation of one FADH2 yields 1.5 ATP; and the oxidation of one acetyl CoA yields 10 ATP. ) Group of answer choices a. 96 ATP b. 92 ATP c. 94 ATP d. 34 ATP e. 36 ATP
- Isomaltose (an isomer of maltose), one of the degradation products of starch is further hydrolyzed into its monosaccharide components prior to its intestinal absorption and entry into the glycolysis. Calculate the no. of ATP molecules produced from the digestion and complete oxidation of 1 molecule of isomaltose considering the glycerol-3-phosphate shuttle. Answer the ff.: a) Total no. of glucose molecules entering glycolysis b) Total no. of pyruvate mols. produced at the end of glycolysis c) Total no. of mitochondrial NADH produced after pyruvate is acted upon by pyruvate dehydrogenase complex d) Total no. of CO2 released right after the pyruvate dehydrogenase complex reaction e) Total no. of acetyl CoA mols. entering the Citric Acid Cycle f) Total no. of net cytosolic ATP mols. produced right after glycolysis g) Total no. of all NADH mols. produced after complete oxidation h) Total no. of all FADH2 mols. produced after complete oxidation i) Total no. of all mitochondrial…Show how the following fatty acid is oxidized:In the context of fatty acid oxidation to acetyl CoA, indicate whether each of the following substances is involved in (1) fatty acid activation, (2) fatty acid trans-port, or (3) b-oxidation pathway. More than one choice may be correct in a given situation. a. AMp b. FAD c. Acyl CoA d. H2O