Determine the sequence of the following heptapeptide: • Amino acid analysis of the heptapeptide revealed that the original peptide was composed of: R, V, Y, E, K, A and G. • Reaction of the heptapeptide with dansyl-Cl and acid hydrolysis gave dansyl-A. • Digestion of the heptapeptide with: • carboxypeptidase gave G as the first detectable amino acid. • trypsin gave free R, a dipeptide (A-K) and a tetrapeptide containing E, G, Y and V • digestion of the tetrapeptide above (derived from the trypsin digestion above) with chymotrypsin gave two dipeptides: V-Y and E-G. • pepsin gave a tetrapeptide and a tripeptide (Y-E-G).
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Determine the sequence of the following heptapeptide:
• Amino acid analysis of the heptapeptide revealed that the original peptide was composed of: R, V, Y, E, K, A and G.
• Reaction of the heptapeptide with dansyl-Cl and acid hydrolysis gave dansyl-A.
• Digestion of the heptapeptide with:
• carboxypeptidase gave G as the first detectable amino acid.
• trypsin gave free R, a dipeptide (A-K) and a tetrapeptide containing E, G, Y and V
• digestion of the tetrapeptide above (derived from the trypsin digestion above) with chymotrypsin gave two dipeptides: V-Y and E-G.
• pepsin gave a tetrapeptide and a tripeptide (Y-E-G).
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