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- Which of the following options shows the correct order of fastest to slowest motion of chloride ions, glycine and proteins through the separating gel? Chloride ions > Proteins > Glycine Glycine > Chloride ions > Proteins Chloride ions > Glycine > Protein Protein > Chloride ions > GlycineYou are given a 27 % (w/v) solution of psicose. Molar mass: psicose (C6H12O6) = 180.156 g/mol. ou have a 0.75 mg/mL solution of bovine serum albumin (BSA). You add 1 mL of the 0.75 mg/mL BSA solution into a standard cuvette (1 cm path length), set your spectrophotometer to 280 nm and read the absorbance of this BSA solution. Molecular mass of bovine serum albumin = 66.4 kDa. Molar extinction coefficient (e) of BSA = 43824 M-1 cm-1 (at 280 nm). What absorbance reading will you obtain?i) Write down the equation derived from your Excel generated standard curve and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).
- Select all that apply, meaning there can be more than one correct answer Common factors affecting menbrane fluidity include a.) Temperature. b.) presence of cholesterol c.) number of double bonds in the fatty acid chains. d.) length of phospholipid fatty acid chains Which of the following methods can seperate proteins based on their mass? a.) centrifugation b.) gel filtration chromatography c.) SDS polyacrylamide gel electrophoresis d.) ion exhange chromatographyWhich unknown solution contains the highest concentration of sucrose? Three lab groups carried out an experiment to identify the concentration of sucrose in six solutions. Each unknown solution (1-6) contained one of the following sucrose concentrations: 0.0 M, 0.2 M, 0.4 M, 0.6 M, 0.8 M, and 1.0 M. Cubes of sweet potato (1 cm3) were soaked for 24 hours in each solution and weighed to determine the change in mass. Each data entry represents the average of three sample replicates expressed as percent change in mass following a 24-hour soak in the unknown solutions. Unknown Percent Change in Mass Percent Change in Mass Percent Change in Mass Group 1 Group 2 Group 3 1 6.6 7.8 7.5 2 3.1 3.7 2.9 3 -2.7 -3.5 -2.5 4 0.7 0.5 1.1 5 -11.6 -12.3 -12.6 6 -5.2 -6.2 -4.9 Which unknown solution contains the highest concentration of sucrose? a) 3 b) 4 c) 5 d) 11.What is the molar concentration of a 15% glycerol solution? (Glycerol has a molecular weight of 92.1.)2.How do you prepare 750 mL of 0.5X gel running buffer from a 5X stock solution?
- Compute for the lactic acid content of these samples given the values below. Show your solution Volume of 0.1 N NaOH in 1st Sampling: 10 mL Volume of 0.1 N NaOH in 2nd Sampling: 40 mL Volume of sample used: 10mlYou receive an order for 750 mL of 27% dextrose solution. You have available D50W, 1000 mL. How many mL of D50W and sterile water are needed to complete the order? *What does translucent spot mean with regards to lipid? Identify the control for this test. 1. Water
- How do I calculate the final concentration? I just need one solution to walk through the steps. Prepare eight standards of glucose with your 1 mM glucose solution, each with a final volume of 10 mL. Complete the table on the next page, indicating what your final concentration of glucose will be for each standard, in mM. Volume of Potassium Ferrocyanide Solution Volume of Enzyme Solution Volume of 1 mM glucose solution Final volume of solution Final Concentration of glucose in final solution (mM) 2.50 mL 1.00 mL 0.00 mL 10.00 mL 2.50 mL 1.00 mL 0.50 mL 10.00 mL 2.50 mL 1.00 mL 1.00 mL 10.00 mL 2.50 mL 1.00 mL 2.00 mL 10.00 mL 2.50 mL 1.00 mL 3.00 mL 10.00 mL 2.50 mL 1.00 mL 4.00 mL 10.00 mL 2.50 mL 1.00 mL 5.00 mL 10.00 mL 2.50 mL 1.00 mL 6.00 mL 10.00 mLCalculate how much agarose is needed to make a 3% agarose gel in a volume of 150 ml 1x TAE buffer.Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…