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1. Why do we usually dilute cells before counting them in a hemocytometer?
2. Describe how a biosafety cabinet provides protection for the product, environment, and yourself.
3. Why are the cells grown in a CO2 incubator?
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- 4. When an organism is positive for the coagulase enzyme, which of the following would be present in the test tubes containing rabbit plasma? A. White precipitate at the bottom B. Gel plug C. Liquid D. Pellicle growth at the top1. The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization? 3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilled1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.
- 1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results. 3. Please provide the scientific name of your microbe that was used in the UV experiment (i.e. S. aureus). Compare your plates and interpret/analyze your results. Please discuss your findings and any patterns you were able to gather. 4. After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to control S. marcescens growth? P. aeruginosa? Please explain why you believe these agent(s) work. 5. What purpose does water serve in the “Effects of Antiseptics & Disinfectants” lab? What did you…1. What is the purpose of an autoclave and what is its mechanism?2. Why is there a need for an incubator in the lab? How about incubating cultures at roomtemperature? How can this affect microbes?3. What are the different hoods in the lab and why are they important?2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)
- 1. In Microbiology, are there technologies that can help make bacterial culture and sensitivity done faster?3. How are aseptic technique similar and different in the lab and healthcare field? Be specific and explain at least two differences and two similarities. 4. You are asked to develop a method to transfer an unknown organism from a liquid broth to a solid petri dish. List each step that you would have to take. Be specific2) A pure culture A) is sterile. B) is a population of identical cells. C) is made of a clearly defined chemical medium. D) contains one microbial cell.
- (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specific2. Why can't we say "sterile" technique? 3. How are aseptic technique similar and different in the lab and healthcare field? Be specific and explain at least two differences and two similarities. 4. You are asked to develop a method to transfer an unknown organism from a liquid broth to a solid petri dish. List each step that you would have to take. Be specific.The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization?3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distille
