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Direct ELISA (Sandwich Method): Positive Result Enzyme-labeled secondary antibody Enzyme Add enzyme- labeled antibodies of known specificity; wash off any that do not bind. Add substrate that changes color when acted upon by enzyme. Add specimen. Antibodies to known antigen attached to well. Enzyme-labeled antibodies of known specificity bind antigen and remain. Color development indicates a positive result. Antibodies in the well "capture" antigen in specimen. (a) Indirect ELISA: Positive Result Add enzyme- labeled anti- human IgG; wash off any antibodies that do not bind. トイア Add patient's serum; wash off antibodies that do not bind. Add substrate that changes color when acted upon by enzyme. Known antigen attached to well. Color development indicates a positive test. Antibodies in serum bind Enzyme-labeled secondary antibodies bind to IgG and remain. to antigen. (b) FIGURE 18.8 Enzyme-Linked Immunosorbent Assay (ELISA) (a) Positive direct ELISA. (b) Positive indirect ELISA.

Direct ELISA (Sandwich Method): Positive Result Enzyme-labeled secondary antibody Enzyme Add enzyme- labeled antibodies of known specificity; wash off any that do not bind. Add substrate that changes color when acted upon by enzyme. Add specimen. Antibodies to known antigen attached to well. Enzyme-labeled antibodies of known specificity bind antigen and remain. Color development indicates a positive result. Antibodies in the well "capture" antigen in specimen. (a) Indirect ELISA: Positive Result Add enzyme- labeled anti- human IgG; wash off any antibodies that do not bind. トイア Add patient's serum; wash off antibodies that do not bind. Add substrate that changes color when acted upon by enzyme. Known antigen attached to well. Color development indicates a positive test. Antibodies in serum bind Enzyme-labeled secondary antibodies bind to IgG and remain. to antigen. (b) FIGURE 18.8 Enzyme-Linked Immunosorbent Assay (ELISA) (a) Positive direct ELISA. (b) Positive indirect ELISA.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In the first panel of part (a), what is the purpose of the antibodies attached to the well?

Direct ELISA (Sandwich Method): Positive Result Enzyme-labeled secondary antibody Enzyme Add enzyme- labeled antibodies of known specificity; wash off any that do not bind. Add substrate that changes color when acted upon by enzyme. Add specimen. Antibodies to known antigen attached to well. Enzyme-labeled antibodies of known specificity bind antigen and remain. Color development indicates a positive result. Antibodies in the well "capture" antigen in specimen. (a) Indirect ELISA: Positive Result Add enzyme- labeled anti- human IgG; wash off any antibodies that do not bind. トイア Add patient's serum; wash off antibodies that do not bind. Add substrate that changes color when acted upon by enzyme. Known antigen attached to well. Color development indicates a positive test. Antibodies in serum bind Enzyme-labeled secondary antibodies bind to IgG and remain. to antigen. (b) FIGURE 18.8 Enzyme-Linked Immunosorbent Assay (ELISA) (a) Positive direct ELISA. (b) Positive indirect ELISA.
Transcribed Image Text:Direct ELISA (Sandwich Method): Positive Result Enzyme-labeled secondary antibody Enzyme Add enzyme- labeled antibodies of known specificity; wash off any that do not bind. Add substrate that changes color when acted upon by enzyme. Add specimen. Antibodies to known antigen attached to well. Enzyme-labeled antibodies of known specificity bind antigen and remain. Color development indicates a positive result. Antibodies in the well "capture" antigen in specimen. (a) Indirect ELISA: Positive Result Add enzyme- labeled anti- human IgG; wash off any antibodies that do not bind. トイア Add patient's serum; wash off antibodies that do not bind. Add substrate that changes color when acted upon by enzyme. Known antigen attached to well. Color development indicates a positive test. Antibodies in serum bind Enzyme-labeled secondary antibodies bind to IgG and remain. to antigen. (b) FIGURE 18.8 Enzyme-Linked Immunosorbent Assay (ELISA) (a) Positive direct ELISA. (b) Positive indirect ELISA.
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