ii) In order to create a western blot, SDS-PAGE is first performed to separate the proteins. After the electrophoretic separation, the proteins are transferred to a nitrocellulose membrane prior to staining with antibodies specific for the protein understudy. Describe how the antibodies could aid in the protein detection process.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question

ii only.........

. Besides agarose gel electrophoresis, there is also SDS-PAGE (sodium dodecyl sulphate-
polyacrylamide gel electrophoresis) used commonly for protein separation.
i)
ii)
Explain the principle of SDS-PAGE.
In order to create a western blot, SDS-PAGE is first performed to separate the
proteins. After the electrophoretic separation, the proteins are transferred to a
nitrocellulose membrane prior to staining with antibodies specific for the protein
understudy. Describe how the antibodies could aid in the protein detection process.
Transcribed Image Text:. Besides agarose gel electrophoresis, there is also SDS-PAGE (sodium dodecyl sulphate- polyacrylamide gel electrophoresis) used commonly for protein separation. i) ii) Explain the principle of SDS-PAGE. In order to create a western blot, SDS-PAGE is first performed to separate the proteins. After the electrophoretic separation, the proteins are transferred to a nitrocellulose membrane prior to staining with antibodies specific for the protein understudy. Describe how the antibodies could aid in the protein detection process.
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Tissue Renewal
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education