DNA fragments cut by restriction enzymes can form two types of ends. What are these ends called? • View Available Hint(s) salvage and sharp ends tails and heads sticky and blunt ends ligase and blunt ends
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- Briefly describe in your own words what DNA barcoding is. Explain how PCR is used in this process.EXPLAIN DNA microarrays: Types, Applications and their futureQ.1. Imagine you are working for a research laboratory and you are asked to help withthe following:a. Cut the following segment of DNA using E. Co R I. Write the sequence offragments clearly and separately. ATTTACGAATTCTTCCAAGAATTCCTAAATGCTTAAGAAGGTTCTTAAGG b. Show sticky ends? How restriction endonuclease are different from the restriction-modification system?
- 1. How can site-specific recombination be used in recombinant DNA technology? Answer and explain comprehensively.Q.No.4. A) Design a oligonucleotide probe for provided gene sequence using all the guidelines for efficient probe designing. ACAACCCCAAGCCTTCAACCACCCCCTTCCCCCAAATTAGAGATCGATCTCAAGAAGAAGAATGGGTTCCGTCTCTCGCTCTTCTTTGGATCAGAAGCTGGCCATGGCAAAGCGCTGCTCCCACGAGGGAGTTGTCGCGGGAGCAAAGGCGGCCGTGGTTGCAACTGTTGCCTCGGCCATTCCTACTTTGGCTAGCGTTAGGATGATCCCATGGGCGAGGTCCTTCCTTAATCCCGCAGCTCAGGCCCTCATCGTTTCATCAGCGGCGGGGGCGGCGTACTTCATAGTTGCGGACAAGACB) Specify the method for Purification of DNA fragment after digestion.(Genetic engineering).Describe two practical applications of recombinant DNA technology
- RECOMBINANT DNA BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE SIGNIFICANCE/PRACTICAL APPLICATIONS OF THIS DNA TECHNOLOGY BY CONSIDERING ETHICAL AND MORAL IMPLICATIONS BEHIND IT. RECOMBINANT DNA EXAMPLE: MODIFIED TRAIT GENE MODIFICATION RECIPIENT ORGANISM FIELDS OF APPLICATION ALSO, WHAT ARE YOUR INSIGHTS? IN 2 TO 3 SENTENCES.Which technique rapidly replicated specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reactionBriefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at your own words.
- Describe at least one important application of DNA technology in each of the following fields: medicine, DNA fingerprinting, and transgenic organisms.VISUAL SKILLS Compare Figure 20.7 with Figure 16.20.How does replication of DNA ends during PCR proceedwithout shortening the fragments each time?What type of enzymes are used to “cut” desired DNA sequences for use in recombinant gene technology experiments? Identify those two enzymes used to cut and paste both genes into the plasmid. Identify all three strategies used in this lab to maximize transformation success. Explain what it means for bacteria to be “competent.” Explains why bacterial competency is this important for this investigation.