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- describe the blue-white colony selection method in the recombinant colony screening, and present a brief argument for its use in biotechnology labs.Which of the following correctly describes a possible scenario during a run of gel electrophoresis with DNA samples?Why can we use temperature sensitive genetic screening to identify particular mutants within a sample?
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.When the genetic materials are improperly replicated and error cannot be corrected the cell proceeds to:What type of experiment would you be most likely to use to demonstrate that protein X was directly phosphorylated by the M-cyclin/CDK pair a flow cytometry experiment a PCR based assay an in vitro assay a fluorescence microscopy experiment
- What benefits are there when using a DNA microarray over a genetic marker such as a STR?Your supervisor asked you to insert a specific stress-tolerant gene into a tissue cultured plant batch. What can be your possible approach? You may only give a flow chart to show your process.describe each step carefully and use your own words. Steps will be like following; I) Primer design for SLIC cloning and the protocol II) Transformation and sequence validation of the clones III) Culture of HEK293T IV) Transduction of HEK293T cells with plasmid V) Western Blotting VI) Immunfluorescence for Confocal Microscopy
- What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the aboveWhy carry out genetic screening at all?Identify 2 or more DNA-based technologies and discuss their combined applications in generating/developing a DNA marker system. You can cite an existing DNA marker for illustration (but not required). Here are the following DNA-based technologies: - DNA Replication and PCR - Restriction Enzymes and Digest - Nucleic acid electrophoresis - Southern Blot and Hybridization - DNA sequencing
