OO HUAWEI Nova 2 PlusDUAL CAMERAestionTo study the function of any gene of interest you would perform the loss and gain of function approaches by either deletingor re-expressing the gene of interest, which of the following can be used to determine and quantify the activity of thegene?redOa. Microscopyd out ofO b.Western blottingO C.PCR/OLAonO d. Gene knockdownO e. DNA hybridizationstionWhich one of the following is NOT correct regarding Bacterial Biosensors?O a. produced by all Pseudomonas speciesedO b. Encoded by LacZ geneout ofO c.Encoded by Lux genesO d. Chemical compounds used for the quantitative assessment of water pollutionO e.In the presence of pollutants the bioluminescent decreases.stionCD4-Pseudomonas Exotoxin Fusion Protein is another biotechnology strategy for HIV therapeutic setting. This constructwill target and kill:Select one:dO a.Any lymphocyteput ofO b. HIV (virus only)O C.Non infected THO d. Infected TH cellsO e.Infected TH cells and HIV virus

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lo study the function of any gene of interest you would perform the loss and gain of function approaches by either deleting or re-expressing the gene of interest, which of the following can be used to determine and quantify the activity of the gene? a. Microscopy b. Western blotting O c. PCR/OLA O d. Gene knockdown O e. DNA hybridization

lo study the function of any gene of interest you would perform the loss and gain of function approaches by either deleting or re-expressing the gene of interest, which of the following can be used to determine and quantify the activity of the gene? a. Microscopy b. Western blotting O c. PCR/OLA O d. Gene knockdown O e. DNA hybridization

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1TYK

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Match each term with the most suitable description. ______ DNA profile a. GMO with a foreign gene ______ Ti plasmid b. alleles commonly have them ______ probes c. unique array of short tandem repeats ______ SNPs d. used to find clones. ______ transgenic e. mouse vs. human ______ genomics f. used in plant gene transfers ______ CRISPR g. based on RNA interference
After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to: a. amplify the DNA. b. convert cDNA into genomic DNA. c. convert cDNA into messenger RNA. d. verify that the desired DNA sequence has been amplified. e. synthesize primer DNA molecules.
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
A set of cells that host various DNA fragments collectively representing an organisms entire set of genetic information is a _________. a. genome b. clone c. genomic library d. GMO
For each of the restriction enzymes listed below:(i) Approximately how many restriction fragmentswould result from digestion of the human genome(3 × 109bases) with the enzyme? (ii) Estimate theaverage size of the pieces of the human genomeproduced by digestion with the enzyme. (iii) Statewhether the fragments of human DNA produced bydigestion with the given restriction enzyme wouldhave sticky ends with a 5′ overhang, sticky endswith a 3′ overhang, or blunt ends. (iv) If the enzymeproduces sticky ends, would all the overhangs on allthe ends produced on all fragments of the humangenome with that enzyme be identical, or not? (Therecognition sequence on one strand for each enzymeis given in parentheses, with the 5′ end written atthe left. N means any of the four nucleotides; R is any purine—that is, A or G; and Y is any pyrimidine—that is, C or T. ^ marks the site of cleavage.)a. Sau3A (^GATC)b. BamHI (G^GATCC)c. HpaII (C^CGG)d. SphI (GCATG^C)e. NaeI (GCC^GGC)f. BanI (G^GYRCC)g. BstYI…
In DNA-hybridization experiments on six species of plants in the genus Vicia, DNA was isolated from each of the six species, denatured by heating, and sheared into small fragments (W. Y. Chooi. 1971. Genetics 68:213–230). In one experiment, DNA from each species and from E. coli was allowed to renature. The graph shows the results of this renaturation experiment. Q. Can you explain why the E. coli DNA renatures at a much faster rate than does DNA from any of the Vicia species?
The accompanying photo shows a sequencing gel from the original study that first sequenced the cystic fibrosis gene (J. R. Riordan et al. 1989. Science 245:1066–1073). From the photo, determine the sequence of the normal copy of the gene and the sequence of the mutated copy of the gene. Identify the location of the mutation that causes cystic fibrosis. (Hint: The CF mutation is a 3-bp deletion.)
1- A genetic analysis of a bacterium reveals the presence of viral DNA. The most probable way this happened was through (a)mutation (b) conjugation (c)transduction (d)transposons (e)transformation 2- Which statemere is tobe regarding GEMC (a)typical cloning vectors involbe plasmids and viruses (b)eukaryotic genes may only be introduced and expressed in eukaryotic microbes such as yeasts (c)horizontal gene transfer methods may be manipulated to introduce new genes (d) expressions of introduced genes can be monitored through the use of marker genes. (e)it is possible for multiple genes may be added to microbes from other sources . plz give answer for both questions asap please
FOR PKU: Draw what you’d expect to see if you were to analyze the gene products by conventional PCR, RT-PCR and Western blot. Would you see abnormally sized gene products? Explain and be sure to include any relevant control(s) that you should include in your experiment.
1. Choose the phrase from the right column that best fitsthe term in the left column.a. transformation 1. requires supplements in medium forgrowthb. conjugation 2. a method for mutagenizing genes inbacterial genomesc. transduction 3. small circular DNA molecule that canintegrate into the chromosomed. lytic cycle 4. the core genes that define a bacterialspecies plus all of the genes unique toindividual strainse. lysogeny 5. transfer of DNA requiring directphysical contactf. episome 6. integration of phage DNA into thechromosomeg. auxotroph 7. infection by phages in which lysis ofcells releases new virus particlesh. pangenome 8. transfer of naked DNAi. gene targeting 9. transfer of DNA between bacteria viavirus particles
You are working in a molecular biology laboratory and are having challenges with your PCR. You decidet todouble-check the PCR protocol programmed into the thermal cycler and discover that the annealing temperature was programmed to be 65 °C instead of 50 °C, as you had intended. What effect would this mistake have on the PCR reaction? Suggest potential solutions
4. You can carry out matings between an Hfr and F−strain by mixing the two cell types in a small patch ona plate and then replica plating to selective medium.This methodology was used to screen hundreds of different cells for a recombination-deficient recA− mutant. Why is this an assay for RecA function? Wouldyou be screening for a recA− mutation in the F− or Hfrstrain using this protocol? Explain.