The following question is related to Restriction Enzymes and RFLP.Using EcoRl, the smallest fragments between DNA sequence A and B were how many base pairs? DNA sequence A:5'3'-3'-ATTGAATTCCGGTTAGCTTTAGAATTCCGCCATATGCGCAATTGGAATTCCDNA sequence B:5'-TAACTTAAGGCCAATCGAAATCTTAAGGCGGTATACGCGTTAACCTTAAGGATTGAATTCCGGTTAGCTTTACAATTCCGCCATAGGCCCAATTGGAATTCCTAACTTAAGGCCAATCGAAATGTTAAGGCGGTATCCGGGTTAACCTTAAGG--3'5'3'5'

Restriction enzymes are restriction endonucleases that have specific restriction sites and…

DNA sequence A: 5' - 3' TAACTTAAGGCCAATCGAAATCTTAAGGCGGTATACGCGTTAACCTTAAGG 3' DNA sequence B: 5' ATTGAATTCCGGTTAGCTTTAGAATTCCGCCATATGCGCAATTGGAATTCC - - ATTGAATTCCGGTTAGCTTTACAATTCCGCCATAGGCCCAATTGGAATTCC TAACTTAAGGCCAATCGAAATGTTAAGGCGGTATCCGGGTTAACCTTAAGG - 3' 5' 3' 5'

DNA sequence A: 5' - 3' TAACTTAAGGCCAATCGAAATCTTAAGGCGGTATACGCGTTAACCTTAAGG 3' DNA sequence B: 5' ATTGAATTCCGGTTAGCTTTAGAATTCCGCCATATGCGCAATTGGAATTCC - - ATTGAATTCCGGTTAGCTTTACAATTCCGCCATAGGCCCAATTGGAATTCC TAACTTAAGGCCAATCGAAATGTTAAGGCGGTATCCGGGTTAACCTTAAGG - 3' 5' 3' 5'

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter7: Gene Expression And Control

Section: Chapter Questions

Problem 2VQ

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Question

The following question is related to Restriction Enzymes and RFLP. Using EcoRl, the smallest fragments between DNA sequence A and B were how many base pairs?

DNA sequence A:
5'
3'
-
3'
-
ATTGAATTCCGGTTAGCTTTAGAATTCCGCCATATGCGCAATTGGAATTCC
DNA sequence B:
5'
-
TAACTTAAGGCCAATCGAAATCTTAAGGCGGTATACGCGTTAACCTTAAGG
ATTGAATTCCGGTTAGCTTTACAATTCCGCCATAGGCCCAATTGGAATTCC
TAACTTAAGGCCAATCGAAATGTTAAGGCGGTATCCGGGTTAACCTTAAGG
-
-
3'
5'
3'
5'

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Step 1: Restriction enzymes

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Step 2: Diagramming given DNA sequence and cutting it with restriction Enzymes

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