“DNMT3L Connects Unmethylated Lysine 4 of Histone H3 to de novo Methylation of DNA,” the authors determined that the DNMT3L protein interacts with several histone proteins.

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Chapter9: Cell Communication
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In the first figure of “DNMT3L Connects Unmethylated Lysine 4 of Histone H3 to de novo Methylation of DNA,” the authors determined that the DNMT3L protein interacts with several histone proteins. Using Figure a and b (from that paper), explain how each of the 4 histones interact with DNMT3L.

a
Wild type
Tag +
Neo
Tag
Exon: 12 34
"I
ATG
56 7 8
H
HH Pgk1-Neo
ATG
| Cre
Y
HHHHHH
MGSR-His-FLAG
9
+
H
+
10 11
H
loxP site
H
12
2 kb
Figure 1 Generation of epitope-tagged Dnmt3L locus and DNMT3L
protein interaction screen in ES cells. a, Targeting of the endogenous mouse
Dnmt3L locus with a replacement cassette that introduces an N-terminal
His6-FLAG epitope tag. The tag was introduced immediately after the first
four amino acids of DNMT3L (Supplementary Fig. S1). The neo resistance
cassette was deleted by transient exposure to Cre recombinase. Mice
homozygous for the Dnmt3LT8 allele are fertile and were used to generate
homozygous Dnmt3LTag/Tag ES cells. The southern blot on the right shows
13
요
8-
6-
5-
Tag +
Neo
- Tag
-Wild type
b
M, x 10-³
83-
62-
47.5-
32.5-
25-
16.5-
6.5-
+/+ Tag/Tag
DNMT3A2
DNMT3B
-DNMT3L
H3
H2A/H2B
-H4
that homologous recombination and Cre-mediated excision of the marker
occurred as predicted. b, Protein interaction screen in wild-type and
Dnmt31 Tag/Tag p
ES cells by FLAG immunoprecipitation. Coomassie-stained
bands were subjected to MALDI-reTOF and mass spectra were screened
against a non-redundant protein database to identify interacting proteins.
The protein band marked by the asterisk is actin, a common contaminant in
anti-FLAG immunoprecipitation.
Transcribed Image Text:a Wild type Tag + Neo Tag Exon: 12 34 "I ATG 56 7 8 H HH Pgk1-Neo ATG | Cre Y HHHHHH MGSR-His-FLAG 9 + H + 10 11 H loxP site H 12 2 kb Figure 1 Generation of epitope-tagged Dnmt3L locus and DNMT3L protein interaction screen in ES cells. a, Targeting of the endogenous mouse Dnmt3L locus with a replacement cassette that introduces an N-terminal His6-FLAG epitope tag. The tag was introduced immediately after the first four amino acids of DNMT3L (Supplementary Fig. S1). The neo resistance cassette was deleted by transient exposure to Cre recombinase. Mice homozygous for the Dnmt3LT8 allele are fertile and were used to generate homozygous Dnmt3LTag/Tag ES cells. The southern blot on the right shows 13 요 8- 6- 5- Tag + Neo - Tag -Wild type b M, x 10-³ 83- 62- 47.5- 32.5- 25- 16.5- 6.5- +/+ Tag/Tag DNMT3A2 DNMT3B -DNMT3L H3 H2A/H2B -H4 that homologous recombination and Cre-mediated excision of the marker occurred as predicted. b, Protein interaction screen in wild-type and Dnmt31 Tag/Tag p ES cells by FLAG immunoprecipitation. Coomassie-stained bands were subjected to MALDI-reTOF and mass spectra were screened against a non-redundant protein database to identify interacting proteins. The protein band marked by the asterisk is actin, a common contaminant in anti-FLAG immunoprecipitation.
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