Please help.I am stuck 0.510410 102 10 1 10' 10² 103³ 10*Cot (mole × sec/L)In 1968, Britten and Kohne showed that once double-stranded DNA is dissociated into single-stranded pieces, the rate of reassociation of the complementary strands can be analyzed. Forthese kinds of experiments, the DNA is first sheared into fragments with an average size ofseveral hundred base pairs. The fragments are dissociated (denatured) via heating, and thenthe sample temperature is lowered slowly while the reassociation is monitored. Duringreassociation, pieces of single-stranded molecules collide at random. If the pieces arecomplementary, a stable double-stranded molecule is formed. If no match is made, the piecesseparate and continue encountering other single-stranded pieces. This process continuesuntil all matches are made.a. The figure below shows an ideal time course for reassociation of DNA. Co is the initialconcentration of DNA single strands in moles per liter. Time (t) of the reassociation ismeasured in seconds. C is the concentration of single-stranded DNA remaining after sometime, t, has elapsed. For simplicity, you can think about the X-axis as time elapsed.i. At time t = 0, what is the relationship of C vs. Co?ii. You can see the slope of the line changes over the span of the reaction. Describe theslope at the beginning, middle and end of the reaction using the following words: steepand shallow.iii. Explain why the slope is as you describe it at the beginning, middle and end of thereaction. Provide a molecular explanation.

All organisms must replicate their DNA when cells divide. During DNA replication, an enzyme called…

Answered: double-stranded DNA is dissociated into

Science

Biology

double-stranded DNA is dissociated into

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna

Section: Chapter Questions

Problem 14QP: State the properties of the WatsonCrick model of DNA in the following categories: a. number of...

See similar textbooks

Related questions

Q: The fragment of DNA in replication are called______fragments.

A: During DNA replication ,double helix is unwound and the complementary strands are separated with the…

Q: Describe the functions of DNA.

A: DNA ( Deoxyribonucleic acid) is a macromolecule which is made up of nucleotides having sugar ,…

Q: A DNA sequence can be represented as a string of the letters ACTG (short for , cytosine, guanine,…

A: The sequence of DNA defines the order of the four chemical building blocks called bases that make up…

Q: The enzyme responsible for adding new nucleotides to a growing DNA chain during DNA replication is…

A: DNA is the nucleic acid that stores genetic information.

Q: Which is the crucial role of base pairing between codon and anticodon

A: The triplet code expresses the genetic information that is transcribed into mRNA. The tRNA translate…

Q: Joining two DNA molecules using DNA ligase. Ligation. O Adhesion. Linker Linkage.

A: DNA fragment is joined with another DNA fragment by the formation of phosphodiester bond. Enzyme…

Q: What holds double-stranded DNA together

A: (The answer to the first question is given. For the second question please repost it as mathematics…

Q: Chemical and Radiation Damage to DNA Can Lead to ____________.

A: Deoxyribonucleic acid (DNA) is a molecule made up of two polynucleotide chains that coil around each…

Q: Replication of DNA begins at and transcription of RNA begins at

A: In DNA replication the double-stranded molecule of DNA is copied for making two identical DNA…

Q: If a DNA strand has the sequence ATGCGATCCGC thenthe sequence on the complementary DNA strand is…

A: Answer is b.) TACGCTAGGCG.

Q: What process allows chromosomes to exchange section of DNA

A: A chromosome is a thread like structure present in the nucleus of the cell. It contains the DNA and…

Q: DNA can copy itself through a process known as _____.

Q: Whole DNA chromosomes are kept in the nucleus while small nucleotide monomers move into and out of…

Q: DNA is a large molecule and is wrapped around proteins called: O a. Histones O b. Ligase O c.…

A: DNA (deoxyribonucleic acid) is the molecule that conveys genetic information for an organism's…

Q: differentiates the DNA from RNA.

A: DNA: It stand for deoxyribonucleic acid, which is a molecule that contains the instructions an…

Q: The bacterial chromosome is a ring of____________-stranded DNA.

A: DNA is the source of genetic information that flows from generation to generation. DNA molecules are…

Q: The _____________ strand is made continously.

A: DNA replication in eukaryotes is semiconservative, semicontinuous, and bidirectional. It occurs in…

Q: RNA polymerases

A: Transcription is the metabolic process where the RNA molecules are synthesis from the antisense…

Q: Describe the synthesis of lagging strand of DNA

A: Replication of DNA is defined as a process by which a double-stranded DNA molecule is copied to give…

Q: Mutations within a DNA sequence are

A: Mutations are errors in the DNA replication process. It may be either due to mistakes when the DNA…

Q: DNA replication results in the creation of _____________ identical strands of DNA.

A: The consequence of DNA replication is two DNA molecules comprising of one new and one old chain of…

Q: An enzyme called _____________________ catalyzes theformation of a covalent phosphodiester bond…

A: DNA replication is the process of formation of complementary strands of DNA molecules from the…

Q: Biological functions of DNA

A: INTRODUCTION DNA is required for heredity, protein-coding, and delivering instructions for life and…

Q: Molecule involved in joining segmented nucleotide strands is... O a. DNA ligase b. Ribosomes c. RNA…

A: DNA is the nucleic acids present in the organisms. DNA is the deoxy-ribose nucleic acid in which…

Q: describe the chemical synthesis of DNA.

A: The process of synthesizing the required gene has many applications in biochemical and…

Q: Once replication begins, it will continue in both ______________ along the DNA strand.

A: Replication Replication is the process of doubling of DNA molecules. In this, both strands of DNA…

Q: The process of forming RNA from DNA is called a. mitosis. b. DNA replication. c. translation. d.…

A: Transcription is the first step involved in gene expression wherein a small DNA segment is copied to…

Q: During DNA Replication, _____________ is synthesized in a series of short pieces called…

A: DNA is made up of various nucleotides that store genetic information in it. DNA is packed into a…

Q: Interesting knowledge about DNA.

A: DNA is involved particles called nucleotides. Each nucleotide contains a phosphate group, a sugar…

Q: _____________ is responsible for adding new nucleotides to the DNA strand being created.

A: Two long polynucleotide chains made up of four diverse nucleotide sub units make up a DNA particle.…

Q: The enzymes responsible for forming the final phosphoiester bond between two DNA fragments during…

A: The phosphodiester bonds constitute the backbone of the strands of DNA.

Q: DNA positively supercoils during replication and negatively supercoils in transcription.

A: Transcription is a process where DNA replicate into RNA, while replication is the making of DNA…

Q: What does DNA code

A: A gene is an essential unit of heredity and an arrangement of nucleotides in DNA (deoxyribonucleic…

Q: DNA replication is bidirectional and discontinuous

A: DNA replication is a DNA dependent DNA synthetic process. DNA acts as the genetic material in the…

Q: DNA polymerase adds nucleotides to the new strand in the ___________ to _____________ direction.

A: At the time of synthesizing new DNA, free nucleotides can be added by DNA polymerase only to the 3'…

Q: Expressed sequences of DNA are called ________________.

A: DNA or deoxyribonucleic acid is a molecule composed of two polynucleotide chains that coil around…

Q: The process whereby DNA molecules are cut and rejoined in new combinations is called _____________ .

A: DNA (deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…

Q: Define the term DNA.

A: Gregor Mendel: Father of genetics Genetic material are the substance which are responsible for the…

Q: . DNA synthesis begins at specific nucleotide sequences, called leading strand, and proceeds in…

A: False DNA synthesis starts at a specific place on a chromosome called an origin of replication.It…

Q: Each new double helix will contain ___________ strand of DNA from the parent strand and ____________…

A: The method of replicating a double-stranded DNA molecule into two identical DNA molecules is known…

Q: the genetic information is coded in DNA by the the sequence of

A: The sequence of DNA bases is arranged into genes, most of which contain the instructions to create a…

Q: Types of DNA inhibitors medicines

A: DNA inhibitors drugs are those chemical formulation that inhibit the DNA and its function.

Q: The Central dogma for biology includes: DNA to RNA which is called

A: Central Dogma is one of the most important concepts in Biology which involves the processes in which…

Q: A double helix is made up of _____________ strands

A: DNA stands for Deoxyribonucleic acid. DNA is a polynucleotide strand. DNA consists of five carbon…

Q: Removes RNA primer and replaces it with newly synthesized DNA.

A: A primer is a single-stranded nucleic acid that all living organisms utilize to start the production…

Q: DNA polymerase

A: For DNA to under replication it requires different proteins and enzymes. They are, DNA polymerases,…

Q: If DNA segments changes from GCATAG to GCATA, this is a:

A: Mutation is a sudden, stable, and variable change in the genome of an organism. It may be due to…

Q: When the dna is relaxed in the cell and is all stringy and evenly spread throughout the nucleus, we…

A: The human DNA of 6.6 x 109 base pairs (bp) is compactly packed using histone proteins inside the…

Q: The molecule is responsible for bringing the code from DNA to the ribosome during protein synthesis.

A: Ans. The messenger RNA (mRNA), in molecular biology, is a single-stranded RNA molecule that matches…

Concept explainers

Immune Disorders

The collaboration between the cells and proteins that work together as a defense system against infection comprises the immune system.Each of them performs a variety of tasks to recognize and/or react against a foreign body.

Question

Please help.I am stuck

0.5
104
10 102 10 1 10' 10² 103³ 10*
Cot (mole × sec/L)
In 1968, Britten and Kohne showed that once double-stranded DNA is dissociated into single-
stranded pieces, the rate of reassociation of the complementary strands can be analyzed. For
these kinds of experiments, the DNA is first sheared into fragments with an average size of
several hundred base pairs. The fragments are dissociated (denatured) via heating, and then
the sample temperature is lowered slowly while the reassociation is monitored. During
reassociation, pieces of single-stranded molecules collide at random. If the pieces are
complementary, a stable double-stranded molecule is formed. If no match is made, the pieces
separate and continue encountering other single-stranded pieces. This process continues
until all matches are made.
a. The figure below shows an ideal time course for reassociation of DNA. Co is the initial
concentration of DNA single strands in moles per liter. Time (t) of the reassociation is
measured in seconds. C is the concentration of single-stranded DNA remaining after some
time, t, has elapsed. For simplicity, you can think about the X-axis as time elapsed.
i. At time t = 0, what is the relationship of C vs. Co?
ii. You can see the slope of the line changes over the span of the reaction. Describe the
slope at the beginning, middle and end of the reaction using the following words: steep
and shallow.
iii. Explain why the slope is as you describe it at the beginning, middle and end of the
reaction. Provide a molecular explanation.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1 Introduction

Step 2 Explanation

Step 3

Step 4 Conclusion

Trending now

This is a popular solution!

Step by step

Solved in 4 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

State the properties of the WatsonCrick model of DNA in the following categories: a. number of polynucleotide chains b. polarity (running in same direction or opposite directions) c. bases on interior or exterior of molecule d. sugar/phosphate on interior or exterior of molecule e. which bases pair with which f. right- or left-handed helix
A duplex DNA molecule contains a random sequence of the four nucleotides with equal proportions of each. What is the average spacing between consecutive occurrences of the sequence 5'-ATGC-3'? Between consecutive occurrences of the sequence 5'-TACGGC-3'?
A single strand of DNA, 24 nucleotides long, with the sequence 5'-TTTCCCgggAAAgggTTTAAAggg-3' is in a test tube. (Note that G's are shown in lowercase, so that your eye can better distinguish them from C's) Other than the appropriate buffer solution, what else needs to go in the test tube to so that we end up with a piece of double stranded DNA, 24 base pairs long, with the above sequence comprising one of the two strands?
5’ TAAGCGTAACCCGCTAA CGTATGCGAAC GGGTCCTATTAACGCAC 3’ 3’ ATTCGCATTGGGCGATT GCATACGCTTG CCCAGGATAATTGCGTG 5’ Imagine that the double-stranded DNA molecule shown above was broken at the sites indicated by spaces in the sequence and that before the breaks were repaired the DNA fragment between the breaks was reversed. What would be the base sequence of the repaired molecule? Show the sequence, label the 5’ and 3’ ends and briefly explain the reasoning supporting your answer
Assuming the average weight of a deoxynucleotide monophosphate (dNMP) is 327.0 g/mol, how many picomoles of DNA are present in 500ng of a 1000bp DNA fragment?
What is the concentration of a DNA solution that absorbs 0.812 and 0.463 at 260 and 280 nm, respectively? Is the DNA solution considered to be good quality? Why or why not?
When a double-stranded DNA molecule is exposed tohigh temperature, the two strands separate, and themolecule loses its helical form. We say the DNA hasbeen denatured. (Denaturation also occurs whenDNA is exposed to acid or alkaline solutions.)a. Regions of the DNA that contain many A–T basepairs are the first to become denatured as the temperature of a DNA solution is raised. Thinkingabout the chemical structure of the DNA molecule, why do you think the A–T-rich regionsdenature first?b. If the temperature is lowered, the original DNAstrands can reanneal, or renature. In addition to thefull double-stranded molecules, some molecules ofthe type shown here are seen when the moleculesare examined under the electron microscope. Howcan you explain these structures?
T. aquaticusgenomic DNA is 34.3% guanosine nucleotides. What fraction of the DNA is adenosine nucleotides?
Agarose gels with different average pore sizes areneeded to separate DNA molecules of different sizeclasses. For example, optimal separation of 1100 bpand 1200 bp fragments would require a gel with alarger average pore size than optimal separation of8500 bp and 8600 bp fragments. How do you thinkthat scientists prepare gels of different average poresizes? (Hint: Agarose gels are made in a mannersimilar to gelatin desserts such as JELL-O.)
The base composition for one of the strands of a DNA double helix is 19% A, 34% C, 28% G, and 19% T. What is the percent base composition for the other strand of the DNA double helix?
if the guanine content of certain segment of double-stranded DNA is 22% what is the adenine content?
When the helix axis of a closed circular duplex DNA of 2310 bp is constrained to lie in a plane, the DNA has a twist (T) of 207. When released, the DNA takes up its normal twist of 10.5 bp per turn. Indicate the values of the linking number (L), writhing number (W), and twist for both the constrained and unconstrained conformational states of this DNA circle. What is the superhelix density, σ = W/T, of both the constrained and unconstrained DNA circles?