a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total number of cells present in a patient’s specimen.b. After performing the streak plate method on a bacterial specimen, theculture was incubated for 48 hours at 37°C. Upon viewing the plate, therewas heavy growth (with no isolated colonies) in the fi rst quadrant, but nogrowth was apparent in the remaining quadrants. Please discuss errors in the procedure that could have produced this result.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.4: The Gram Stain
Problem 5RQ
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a. Explain whether or not any of the methods in fi gure 2.9 could be
used to determine the total number of cells present in a patient’s specimen.
b. After performing the streak plate method on a bacterial specimen, the
culture was incubated for 48 hours at 37°C. Upon viewing the plate, there
was heavy growth (with no isolated colonies) in the fi rst quadrant, but no
growth was apparent in the remaining quadrants. Please discuss errors in the procedure that could have produced this result.

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