Concepts of Biology
1st Edition
ISBN: 9781938168116
Author: Samantha Fowler, Rebecca Roush, James Wise
Publisher: OpenStax College
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Textbook Question
Chapter 10, Problem 4RQ
What carries a gene from one organism into a bacteria cell?
a. a plasmid
b. an electrophoresis gel
c. a restriction enzyme
d. polymerase chain reaction
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What do genetic engineers use to create the “sticky ends” needed to splice two fragments of DNA together?
a.) an amino acid sequence
b.) DNA ligase
c.) restriction enzymes
d.) mRNA
Which technique allows a researcher to change a single specific nucleotide in a gene sequence in vitro?
a.
The CRISPR-Cas9 system
b.
PCR-based random mutagenesis
c.
PCR-based site-specific mutagenesis
d.
RT-PCR
Choose the combination of answers that most accurately completes the statement. Which gene is incorporated into plasmids to detect recombinant cells? a. restriction endonuclease c. a gene for antibiotic resistance b. virus receptors d. reverse transcriptase
Chapter 10 Solutions
Concepts of Biology
Ch. 10 - Figure 10.7 Why was Dolly a Finn-Dorset and not a...Ch. 10 - n gel electrophoresis of DNA, the different bands...Ch. 10 - In the reproductive cloning of an animal, the...Ch. 10 - What carries a gene from one organism into a...Ch. 10 - What is a genetically modified organism (GMO)? a....Ch. 10 - What is the role of Agrobacterium tumefaciens in...Ch. 10 - What is the most challenging issue facing genome...Ch. 10 - Genomics can be used in agriculture to: a....Ch. 10 - What kind of diseases are studied using...Ch. 10 - What is the purpose and benefit of the polymerase...
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- separates molecules by movement due to size and electrical charge [ Choose ] a. DNA ligase b. gene cloning c. gel electrophoresis d. restriction enzymesarrow_forwardWhat is the role of Proteinase K in DNA isolation? A. Inhibit enzymes B Catalyze breakdown of nuclei acids C Degrade proteins D Remove disultide bongs in proteinsarrow_forwardWhich gene is incorporated into plasmids to detect recombinantcells?a. restriction endonuclease b. virus receptors c. a gene for antibiotic resistance d. reverse transcriptasearrow_forward
- Why must a genetically engineered plasmid contain a genetic marker? a.) to prevent the construction of an artificial chromosome b.) to separate cells that contain recombinant DNA from those that do not c.) to produce multiple copies of the recombined plasmid after heat treatment d.) to break apart the circular plasmid and introduce another DNA fragmentarrow_forwardWhich process consists of modifying an individual’s genome? a. DNA profiling b. SNPing c. genetic engineering d. DNA hybridization e. PCRarrow_forwardSecond part asks: Explain the roles two of those enzymes would play in obtaining the recombinant plasmidsarrow_forward
- Give a reason why a plasmid is useful for DNA transferarrow_forward**What are the scientific applications of restriction enzymes? Write about 15 to 20 pointsarrow_forwardWhat is the role of Polyvinylpyrrolidone in DNA isolation? A. Cell lysis B Remove polyphenols C Inhibit enzymes D Create an aqueous layer where DNA staysarrow_forward
- _____ can fix a genetic flaw in a human. a. Cloning b. Electrophoresis c. Sequencing d. Gene therapyarrow_forwardWhich helps prevent errors in DNA replication? A) Complementary base pairing reduce errors B) DNA ligase checks the DNA for errors C) DNA is located in the ribosomes D) Any base can pair with any other basearrow_forwarda) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c) Compare and contrast the these enzymes in nature and in scientific research.arrow_forward
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