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- CYP2E1 has a much higher Km for ethanol than ADH (11 mM versus 0.05 mM,respectively). When greater portions of ethanol are ingested (0.08% or 17 mM), which part of the statement would be true?1.A patient with chronic pancreatitis and hence decreased secretory function of pancreas was prescribed an enzymatic drug «Enzystal» before each meal. Explain this prescription. For that: a)Name pansreatic enzymes, participating in digestion of carbohydrates, lipids and proteins. What is their pH optimum and how is it reached in duodenum? b) Draw the scheme representing the cascade of activation of pancreatic proteolytic enzymes. Indicate the mechanism ofactivation and its reversability c)Why are proteolytic enzymes secreted by pancreas in inactive form? d)What recommendations on diet can be given to this patient?What is the typical composition of Harris Hematoxylin Solution? Which cellular organelle was more visible after immersing the intestinal cells in hematoxylin?
- It has been shown that pathogenic microbes that invade and reside within macrophages require the ability to prduce catalse and like enzymes, why?Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?a) At what pH will you try to bind lysozyme to a cation exchanger? b) Is it possible to perform this binding even at a different pH than the one you mentioned in section a? Explain your answer. c) Is it possible to bind lysozyme to an anion exchanger as well? If so, at what pH?
- 1) Draw a chromatogram depicting separation of proteins 1, 2, and 3 (protein #1, mw 30,000, protein #2, mw 60,000, and protein #3, mw 90,000). b)Indicate on the chromatogram in (a) where insulin would elute. Would insulin have total accessibility, some accessibility or no accessibility to the particle pores on this column? c)Referring to the size exclusion chromatogram in (a), indicate on the chromatogram where you would expect a molecule with a size of 40,000 to elute. Would you expect the molecule to be well resolved, somewhat resolved, or not resolved from the peak for protein #1?The transport of aspirin (pKa = 3.5, structure shown here) from the digestive tract to the circulation occurs by nonmediated absorption into cells lining the stomach (where pH = 0.8) and the small intestine (where pH 6.0). Do you expect absorption to be faster in the stomach or in the small intestine?(human physiology) The answers to the sentences 15.1,2,3,4,5 and 16.1,2,3,4 are:15.1 - 4000Hz15.2 - A15.3 - B15.4 - D15.5 - C16.1 - F16.2 - T16.3 - 0.25ms16.4 - 10000How to explain these answers?
- Using the graph data I estimated Slope = 2.8, about the same as the steeper part of the maternal hemoglobin, does this represent a strong positive cooperativity between enzyme subunits?In the isolation of glycogen from liver, why it is necessary to filter the solution while it is still hot? Explain in 1-3 sentencesWhich step of cellular respiration is 6Co2 used at?