Experiment 1: Starch Digestion by Amylase Tube number A1 A2 A3 A4 Contents amylase, water Lugol’s Solution test result (color) Positive (+) or Negative (-) for starch digestion N/A, control N/A, control N/A, control Benedict’s Reagent test result (color) Positive (+) or Negative (-) for starch digestion N/A, control N/A, control N/A, control Other comments or possible errors? Experiment 1: Starch Digestion by Amylase Note on safety: goggles should be worn during the entire set of 3 experiments until clean up is complete. Label 4 test tubes A1, A2, A3, and A4. (“A” stands for amylase). To each of the test tubes, add the following: (20 drops = 1 mL). Test tube A1: 1 mL (1000 ml) amylase, 1 mL (1000 ml) distilled water Test tube A2: 1 mL (1000 ml) starch solution (shake the starch bottle before adding any, as starch grains tend to settle), 1 mL (1000 ml) distilled water Test tube A3: 1 mL (1000 ml) glucose solution, 1 mL (1000 ml) distilled water Test tube A4: 1 mL (1000 ml) amylase, 1 mL (1000 ml) starch solution Place all tubes in a 37 degree Celsius water bath for 1 hour. Proceed to experiment 2. After one hour, obtain a spot plate. Transfer one drop from each test tube to exactly one spot on the spot plate. Use a different dropper for each tube. To each drop, add one drop of Lugol’s solution, which contains IKI, an iodine salt. A color change to black, blue, or dark brown indicates the presence of starch. If starch is not present, iodine will dye the solution a yellow color Record your results in the RESULTS section. Into the remaining mixture in each test tube, add 3 drops of Benedict’s reagent and place each tube in a beaker of boiling water with 1 or 2 boiling chips for 1 minute. A color change to green, orange, or red indicates sugars are present. If sugars are not present, Benedict’s reagent remains a light blue color Record your results in the RESULTS section.

Experiment 1: Starch Digestion by Amylase Tube number A1 A2 A3 A4 Contents amylase, water Lugol’s Solution test result (color) Positive (+) or Negative (-) for starch digestion N/A, control N/A, control N/A, control Benedict’s Reagent test result (color) Positive (+) or Negative (-) for starch digestion N/A, control N/A, control N/A, control Other comments or possible errors? Experiment 1: Starch Digestion by Amylase Note on safety: goggles should be worn during the entire set of 3 experiments until clean up is complete. Label 4 test tubes A1, A2, A3, and A4. (“A” stands for amylase). To each of the test tubes, add the following: (20 drops = 1 mL). Test tube A1: 1 mL (1000 ml) amylase, 1 mL (1000 ml) distilled water Test tube A2: 1 mL (1000 ml) starch solution (shake the starch bottle before adding any, as starch grains tend to settle), 1 mL (1000 ml) distilled water Test tube A3: 1 mL (1000 ml) glucose solution, 1 mL (1000 ml) distilled water Test tube A4: 1 mL (1000 ml) amylase, 1 mL (1000 ml) starch solution Place all tubes in a 37 degree Celsius water bath for 1 hour. Proceed to experiment 2. After one hour, obtain a spot plate. Transfer one drop from each test tube to exactly one spot on the spot plate. Use a different dropper for each tube. To each drop, add one drop of Lugol’s solution, which contains IKI, an iodine salt. A color change to black, blue, or dark brown indicates the presence of starch. If starch is not present, iodine will dye the solution a yellow color Record your results in the RESULTS section. Into the remaining mixture in each test tube, add 3 drops of Benedict’s reagent and place each tube in a beaker of boiling water with 1 or 2 boiling chips for 1 minute. A color change to green, orange, or red indicates sugars are present. If sugars are not present, Benedict’s reagent remains a light blue color Record your results in the RESULTS section.

Nutritional Sciences: From Fundamentals to Food, Enhanced Edition

3rd Edition

ISBN:9781337486415

Author:McGuire

Publisher:McGuire

Chapter2: Nutritional Assessment And Dietary Planning

Section: Chapter Questions

Problem II4DWPA

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Question

RESULTS

Experiment 1: Starch Digestion by Amylase

Tube number	A1	A2	A3	A4
Contents	amylase, water
Lugol’s Solution test result (color)
Positive (+) or Negative (-) for starch digestion	N/A, control	N/A, control	N/A, control
Benedict’s Reagent test result (color)
Positive (+) or Negative (-) for starch digestion	N/A, control	N/A, control	N/A, control
Other comments or possible errors?

Experiment 1: Starch Digestion by Amylase

Note on safety: goggles should be worn during the entire set of 3 experiments until clean up is complete.
Label 4 test tubes A1, A2, A3, and A4. (“A” stands for amylase).
To each of the test tubes, add the following: (20 drops = 1 mL).

Test tube A1: 1 mL (1000 ml) amylase, 1 mL (1000 ml) distilled water

Test tube A2: 1 mL (1000 ml) starch solution (shake the starch bottle before adding any, as starch grains tend to settle), 1 mL (1000 ml) distilled water

Test tube A3: 1 mL (1000 ml) glucose solution, 1 mL (1000 ml) distilled water

Test tube A4: 1 mL (1000 ml) amylase, 1 mL (1000 ml) starch solution

Place all tubes in a 37 degree Celsius water bath for 1 hour. Proceed to experiment 2.
After one hour, obtain a spot plate. Transfer one drop from each test tube to exactly one spot on the spot plate. Use a different dropper for each tube. To each drop, add one drop of Lugol’s solution, which contains IKI, an iodine salt. A color change to black, blue, or dark brown indicates the presence of starch. If starch is not present, iodine will dye the solution a yellow color Record your results in the RESULTS section.
Into the remaining mixture in each test tube, add 3 drops of Benedict’s reagent and place each tube in a beaker of boiling water with 1 or 2 boiling chips for 1 minute. A color change to green, orange, or red indicates sugars are present. If sugars are not present, Benedict’s reagent remains a light blue color Record your results in the RESULTS section.

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