A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: Replicate | Amount of product liberated over 10 minutes (pmol) Concentration of total protein in worm extract (µg/ml) Locality |Hokkaido 1 101 4 87 107 3 3 4 4 88 3 5 89 5 Hon-shu 126 120 1 3 3 156 4 153 119 4 5 |5 4 Kyu-shu 1 103 4 99 5 3 94 4 108 3 5 110 | Okinawa 88 3 2 3 90 4 96 4 5 100 112 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean ± standard error (n=5).
A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: Replicate | Amount of product liberated over 10 minutes (pmol) Concentration of total protein in worm extract (µg/ml) Locality |Hokkaido 1 101 4 87 107 3 3 4 4 88 3 5 89 5 Hon-shu 126 120 1 3 3 156 4 153 119 4 5 |5 4 Kyu-shu 1 103 4 99 5 3 94 4 108 3 5 110 | Okinawa 88 3 2 3 90 4 96 4 5 100 112 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean ± standard error (n=5).
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
Question
Transcribed Image Text:A laboratory in Japan performed a similar experiment on earthworms from different localities
of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical
features, they were interested in geographical differences in the ability of the worms to
breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was
similar to the one you used, except that enzyme activity was measured from 100 µl of worm
extract. Data obtained are displayed below:
Locality
Replicate Amount of product
Concentration of total
protein in worm extract
(µg/ml)
liberated over 10
minutes (pmol)
Hokkaido
1
101
4
87
3
3
107
4
4
88
3
5
89
Hon-shu
1
126
2
2
120
3
3
156
4
4
153
5
5
119
4
Куu-shu
1
103
4
2
99
5
3
94
4
108
3
5
110
5
Okinawa
1
88
3
2
90
4
3
96
4
100
112
5
5
3
From the data in the table, calculate the specific activity of polyphenol oxidase (in units of
umol/min/mg protein) from each locality, presenting the data as mean + standard error (n=5).
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