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Explain how the same gen can be highly expressed in several distinct tissues (e.g. brain, liver, and lung) of an organism, yet silent in all other tissues. Hint: The answer is not that they share the same tissue-specific transcription factors..
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- "In most differentiated tissues, daughter cells retain a memory of gene expression patterns that were present in the parent cell through mechanisms that do not involve changes in the sequence of their genomic DNA" is true or false.Give and briefly explain two examples of how gene expression may be repressed without altering the gene-coding sequence.Given the following schematic for a gene and its associated regulatory regions, answer the following questions by placing the correct letter in the provided blanks please put in the correct letter for the questions What region would provide cell type-specific expression of genes? region What site would significantly increase gene expression rates? = region What region or regions of this gene’s coding sequence are expressed as amino acids = region
- Using coat color in mice and the development of female honeybees as examples, explain how dietary factors can cause epigenetic modifications, leading to phenotypic effects.Classify the following genes as proto-oncogenes or tumor-suppressor genes: p53, ras, Bcl-2, telomerase, jun, andHow might a point mutation in DNA affect the phenotype. How the genome is expressed in the organism? (Please explain this at the molecular level).
- A geneticist uses a generic engineering approach to mutate the TATA-building protein (TBP) of a murine cell line that ordinarily produces high amounts of the LNS protein. The mutation prevents the binging of the TBP to the TATA box. What would be the effect on the cell line that possesses the mutation, especially LNS protein levels?In a few sentences, describe how p53 guards the genome. Include at least two specific ways that p53 guards the genome. In a few more sentences, describe the structure and function of the p53 protein. Structure: what domains are present in the p53 protein? Do p53 work as a single protein or as part of a complex? Function: what do the different domains do? How can p53 do so many different things?Describe the functions of the RAG1 and RAG2 and NHEJ proteins.
