Eye solution absobs Ight of wavelength 480 nm, and for this absorption, the molar extinction coefficient is nol cm-. A sample of the dye of unknown concentration is placed in a cuvette of path length 1 cm and the sorbance reading is 0.23. Calculate the concentration of the solution. D0 1.3 x 10 M Db.1.3 x 10 mM DC1.3 pM Dd7.6 x 10 M e 1.3 x 10 uM

Eye solution absobs Ight of wavelength 480 nm, and for this absorption, the molar extinction coefficient is nol cm-. A sample of the dye of unknown concentration is placed in a cuvette of path length 1 cm and the sorbance reading is 0.23. Calculate the concentration of the solution. D0 1.3 x 10 M Db.1.3 x 10 mM DC1.3 pM Dd7.6 x 10 M e 1.3 x 10 uM

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter15: Molecular Luminescence Spectrometry

Section: Chapter Questions

Problem 15.15QAP: The following lifetimes were measured for the chloride quenching of quinine sulfate given in Example...

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The following lifetimes were measured for the chloride quenching of quinine sulfate given in Example 15-1.The fluorescence intensities are given in the example. (a) Plot fluorescence intensity versus [Cl-]. (b) Plot the ratio of intensity to lifetime, F- versus [Cl-]. (c) Develop a normalization factor to correct the measured fluorescence intensity to that of the solution without quencher. (d) Plot on the same graph Fversus [Cl-] and Fcorr versus (CI-].
Use the data in Figure 4.8 to estimate the ratio of radiation intensity at 10,000 Å (infrared) to that at 5000 Å (visible) from a blackbody at 5000 K. How will this ratio change with increasing temperature? Explain how this change occurs.
A solution was prepared from an amount n = (0.500 ± 0.005) mol dissolved in a volume V = (250.0 ± 0.5) cm3. What is the absolute uncertainty in the concentration in units of mol dm-3?
The molecu le 2,2'-bipyridine (1, bpy) forms a complex with the Ru2+ ion. Ruthenium(ll) tris-(2,2'-bipyridyl), Ru(bpy)3,2+(2), has a strong electronic absorpt ion at 450 nm . The quenching of the * Ru(bpy)3 2+ excited state by Fe(OH2)63+. in acidic solution was monitored by measuring excited state lifetimes at 600 nm. Determine the quenching rate constant for this reaction from the following data:
A student conducted an experiment to determine the concentration of benzene in an ethanol solution. Use the UV spectrum of the solution in a 2.0 cm cell. Within the spectrum, there was a peak attributed to benzene at 260 nm, with an absorption of 0.69. In a reference source, the student found that the adsorption of the benzene strip with ethanol was 2.95 x 10-3 ppm cm-. What is the molar concentration of benzene in the sample (mm of benzene = 78.11 g/mol)
5.4.5 Chemical microanalysis5.4.5.1 Exploitation of characteristic X-raysElectron probe microanalysis (EPMA) of bulk samples is now a routine technique for obtaining rapid, accurate analysis of alloys. A small electron probe (100 nm diameter) is used to generate X-rays from a defined area of a polished specimen and the intensity of the various characteristic X-rays measured usingeither wavelength-dispersive spectrometers (WDS) or energy-dispersive spectrometers (EDS). Typically the accuracy of the analysis is š0.1%. One of the limitations of EPMA of bulk samples is that the volume of the sample which contributes to the X-ray signal is relatively independent of the size of theelectron probe, because high-angle elastic scattering of electrons within the sample generates X-rays. The consequence of this is that the spatial resolution of EPMA is no better than ¾2 µm. In the last few years EDX detectors have been interfaced totransmission electron microscopes which are capable of operating…
The following data were obtained on the initial rates of reaction of a d-metal complex in aqueous solution. For the experiments a. [Y] = 2.7 mmol/dm3, and for experiments b. [Y] = 6.1 mmol/dm3 Conc. complex/(mmol dm-3) 8.01 9.22 12.11 Exp. a. v/ (mol dm-3 s-1) 125 144 190 Exp. b. v/ (mol dm-3 s-1) 640 730 960 b) use the data above to calculate the rate constant. k = ________ (mol/L/s, s-1, L/mol/s, or L2/mol2/s). 3 s.f. normal format.
A Dubosq colorimeter consists of a cell of fixed path length and a cell of variable path length. By adjusting the length of the latter until the transmission through the two cells is the same. the concentration of the second solution can be inferred from that of the former. Suppose that a plantdye of concentration 25 μg dm-3 is added to the fixed cell. the length of which is 1.55 em. Then a solution of the same dye. but of unknown concentration. is added to the second cel l. It is found that the same t ransmittance is obtained when the length of the second cel l is adjusted to 1.18 em. What is the concentration of the second solution?
A substance is known to have a molar extinction coefficient of 18,300mlxcm-1xmg-1 at its wavelength of maximum absorption. The absorption of a solution containing this substance in a 1-cm sample cell was found to be 0.327 at this same wavelength. Determine the concentration of the solution.
The following calibration data were obtained by an atomic absorption spectrophotometric (AAS) method for the determination of mercury in an herbal product. (show complete solution) 1. What is the detection limit for the method? 2. What is the limit of quantitation for the method?
What is the molar extinction coefficient if the pathlength of the sample cell is 5.6mm and the absorbance of 62.5 M solution is 0.222? 6.342X10-3 L mole- cm- 6.342X10-3 L mole- mm- 6.342X10-4 L mole- mm- 6.342X10-4 L mole- cm-
. A student collects the following data for the absorbance of a complex measured in 1.00 cmspectrophotometric cells. The molar absorptivity (ε) of the complex under investigation is 2.25×103L/mol·cm. Calculate the concentration of the complex in solution at each time of the reaction.Time (min)MeasuredAbsorbanceComplex Concentration(M)0.000 1.50010.000 1.31320.000 1.12530.000 0.93840.000 0.750