For another project, you designed primers 1 and 2 with the sequences shown below. Your lab partner suggests you calculate the primer melting temperatures (Tm) using the formula: Tm = [(A+T) x 2°C] + [(G+C) x 4°C]. Calculate the Tm for Primers 1 and 2 and give your answers in the boxes provided below. You must give your answer as a whole number (no decimal places). Units (°C) are already shown so must not be included in your answer. Primer 1 (forward): 5' TTCGTCTGGTGAACTCAG 3' °C Primer 2 (reverse): 5' CAGGGTATGTCCGTATAT 3' °C
For another project, you designed primers 1 and 2 with the sequences shown below. Your lab partner suggests you calculate the primer melting temperatures (Tm) using the formula: Tm = [(A+T) x 2°C] + [(G+C) x 4°C]. Calculate the Tm for Primers 1 and 2 and give your answers in the boxes provided below. You must give your answer as a whole number (no decimal places). Units (°C) are already shown so must not be included in your answer. Primer 1 (forward): 5' TTCGTCTGGTGAACTCAG 3' °C Primer 2 (reverse): 5' CAGGGTATGTCCGTATAT 3' °C
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter13: Artifacts And Noise In Medical Instrumentation
Section: Chapter Questions
Problem 7P
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