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For protein X, you do not know the extinction coefficient of the substrate to calculate the true concentration.  Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0 minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial velocity, which will be expressed as min-1.

For protein X, you do not know the extinction coefficient of the substrate to calculate the true concentration.  Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0 minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial velocity, which will be expressed as min-1.

Principles of Modern Chemistry
Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter18: Chemical Kinetics
Section: Chapter Questions
Problem 75AP
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For protein X, you do not know the extinction coefficient of the substrate to calculate the true concentration.  Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0 minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial velocity, which will be expressed as min-1. 

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