For your 25ul ligation reaction: You need to add 160ng of your digested insert DNA (which is at a concentration of 160ng/ul) and 25ng of your digested plasmid DNA (which is at a concentration of 10ng/ul) to your ligation reaction. You also want 1X ligase buffer (it is provided at a 10X concentration) and 1ul ligase. How much water will you need to add to the reaction? O no water needs to be added O 3.5ul O 16.5ul O 18ul

For your 25ul ligation reaction: You need to add 160ng of your digested insert DNA (which is at a concentration of 160ng/ul) and 25ng of your digested plasmid DNA (which is at a concentration of 10ng/ul) to your ligation reaction. You also want 1X ligase buffer (it is provided at a 10X concentration) and 1ul ligase. How much water will you need to add to the reaction? O no water needs to be added O 3.5ul O 16.5ul O 18ul

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter29: Transcription And The Regulation Of Gene Expression

Section: Chapter Questions

Problem 10P

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Similar questions

I have this strain of e coli. Is P+ o+ Z+ Y+ / I- P+ oC Z- Y+ Will beta-galactosidase and permease be expressed? If they are will they be inducible or constitutive?
For the given genotypes (associated with the lac operon in E. coli), indicate with a "+" or "-" whether functional B - galactosidase would be expected to be produced in the indicated conditions, assuming low glucose levels. B - galactosidase production Genotype No lactose With lactose a) I- O+ Z+ / F' I+ O+ Z+ b) IS O+ Z+/ F' I+ O+ Z+
Plz answer ASAP. I will thumb up You are studying the regulation of the lactose operon in Escherichia coli, by measuring expression of the lacZ gene (i.e production of beta-galactosidase).(a) You identify several loss-of-function mutations in which lacZ is never expressed, in the presence and absence of glucose and lactose. What components of the lac operon could be mutated to produce this phenotype? List all possibilities.
Effects of BPA on phosphorylation of MAPKfamily in RAW264.7 cells conclusion
True/false? if false, justify brieflyBacterial operons gather open-reading-frames that are coupled for both transcription and translation.
The trp operon vs. the lac operon: Which one of these is activated in the presence of a substance, and which is activated in the absence, and why the difference? In other words, what if the cell could think, what would be its rationale for why one is activated in the presence and the other in the absence of something? (Ignore the effects of glucose in the lac operon.)
94In the arrested state (7-14 nucleotides) of elongation, ( ) is required to reactivate transcription. 95C. elegans has about 19,000 genes, a significant number of which are organized into operons. Yesorno 96Transcription activator proteins have at least two independently folding domains: 1. DNA binding domain and 2.
Gal 4 is involved in the regulation of galactose metabolism. Describe how transcription would be affected in the presence of a mutation that resulted in an inability of Gal80 to enter the nucleus?
The symbols a, b, and c in the table below represent theE. coli lac system genes for the repressor (I ), the operator(O) region, and the β-galactosidase (Z), although notnecessarily in that order. Furthermore, the order inwhich the symbols are written in the genotypes is notnecessarily the actual sequence in the lac operon.Activity (+) or inactivity (−) of Z geneGenotype Inducer absent Inducer presenta- b+ c+ + +a+ b+ c- + +a+ b- c- - a+ b- c+/a- b+ c- + +a+ b+ c+/a- b- c- - +a+ b+ c-/a- b- c+ - +a- b+ c+/a+ b- c- + +a. Which symbol (a, b, or c) represents each of the lacgenes I, O, and Z ?b. In the table, a superscript minus sign on a gene symbolmerely indicates a mutant, but some mutant behaviorsin this system are given special mutant designations.Using the conventional gene symbols for the lac operon,designate each genotype in the table.
Suppose that a new mutation lacIes, ('es' stands for ‘extra-strength’) has been discovered in the lac operon that allows the lac-repressor to bind even to the lacOc operator. Other properties of lacIes repressor remain normal (that is the same as of lacI+). Given this genotype Is P+ Oc Z+ Y- / Ies P+ O+ Z+ Y+ , under which conditions will the gene lacZ be transcribed to produce β-galactosidase and the gene lacY transcribed to produce permease? A) With lactose, β-galactosidase and permease are produced, without lactose they are not produced B) With lactose, β-galactosidase is produced, without lactose it is not; permease is never produced C) β-galactosidase is always produced and permease is never produced D) With lactose, β-galactosidase and permease are produced, without lactose they are also produced E) β-galactosidase is always produced and permease is produced only in the presence of lactose
By using separate labelled diagrams, describe as detail as possile the actions of the normal and mutated lac operon in absence of lactose.
True or False? Eukaryotic genomes are organized into operons; each operon consists of a series of genes which code for enzymes involved in a metabolic pathway, under the transcriptional control of a single promoter sequence .